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FE elisa kit :: Rat Ferritin ELISA Kit

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Catalog # MBS722921
Unit / Price
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  48-Strip-Wells-(Competitive)  /  $440 +1 FREE 8GB USB
  48-Strip-Wells-(Sandwich)  /  $440 +1 FREE 8GB USB
  96-Strip-Wells-(Competitive)  /  $640 +1 FREE 8GB USB
  96-Strip-Wells-(Sandwich)  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells-(Competitive)  /  $2,895 +3 FREE 8GB USB
  5x96-Strip-Wells-(Sandwich)  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells-(Competitive)  /  $5,415 +6 FREE 8GB USB
  10x96-Strip-Wells-(Sandwich)  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Ferritin, ELISA Kit

Popular Item
Full Product Name

Rat Ferritin ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sandwich ELISA Manual Insert
Sandwich ELISA PDF Manual
Request for Current Manual Insert
OMIM
gene 615517
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of FE. No significant cross-reactivity or interference between FE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FE and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
25-500ng/mL
Sensitivity
1.0ng/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for FEpurchase
MBS722921 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ferritin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FE. The ELISA analytical biochemical technique of the MBS722921 kit is based on FE antibody-FE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FE elisa kit
Intended Uses: This FE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: FE ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FE. Standards or samples are then added to the microtiter plate wells and FE if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FE present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FE are added to each well to "sandwich" the FE immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FE and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FE concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FE elisa kit

Typical Standard Curve/Testing Data of FE elisa kit
FE elisa kit Typical Standard Curve/Testing Data image
NCBI/Uniprot data below describe general gene information for FE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,226 Da
NCBI Official Full Name
ferritin
NCBI Official Synonym Full Names
ferritin, heavy polypeptide 1
NCBI Official Symbol
NCBI Official Synonym Symbols
FHC; FTH; HFE5; PLIF; FTHL6; PIG15
  [Similar Products]
NCBI Protein Information
ferritin heavy chain; apoferritin; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein
UniProt Protein Name
Ferritin heavy chain
UniProt Synonym Protein Names
Cell proliferation-inducing gene 15 proteinCleaved into the following chain:Ferritin heavy chain, N-terminally processed
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
FTH; FTHL6; OK/SW-cl.84; PIG15; Ferritin H subunit  [Similar Products]
UniProt Entry Name
FRIH_HUMAN
NCBI Summary for FE
This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for FE
Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase; EC 1.16.3.1

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: ferritin complex; mitochondrion; cytosol; nucleus

Molecular Function: ferroxidase activity; protein binding; ferric iron binding; iron ion binding

Biological Process: receptor-mediated endocytosis; negative regulation of cell proliferation; negative regulation of fibroblast proliferation; cellular iron ion homeostasis; immune response; post-Golgi vesicle-mediated transport; transmembrane transport; iron ion transport; intracellular sequestering of iron ion

Disease: Hemochromatosis, Type 5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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