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FGF2 elisa kit :: Bovine fibroblast growth factor 2 (basic) ELISA Kit

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Catalog # MBS921128
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $435 +1 FREE 8GB USB
  96-Strip-Wells  /  $625 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,595 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,905 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

fibroblast growth factor 2 (basic) (FGF2), ELISA Kit

Popular Item
Full Product Name

Bovine Heparin-binding growth factor 2, FGF2 ELISA Kit

Product Synonym Names
Bovine Heparin-binding growth factor 2 (FGF2) ELISA kit; BFGF; FGFB; HBGF-2; basic fibroblast growth factor bFGF; fibroblast growth factor 2; heparin-binding growth factor 2; prostatropin; fibroblast growth factor 2 (basic)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS921128 Testing Data
3D Structure
ModBase 3D Structure for P03969
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine FGF2. No significant cross-reactivity or interference between Bovine FGF2 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
6.25 pg/ml-400 pg/ml
Sensitivity
1.56 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Angiogenesis
Protein Biological Process 3
Angiogenesis
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FGF2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFGF2purchase
MBS921128 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fibroblast growth factor 2 (basic) (FGF2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FGF2. The ELISA analytical biochemical technique of the MBS921128 kit is based on FGF2 antibody-FGF2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FGF2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FGF2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FGF2 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of FGF2 elisa kit
FGF2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS921128. Click to request current manual
NCBI/Uniprot data below describe general gene information for FGF2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
17,250 Da
NCBI Official Full Name
fibroblast growth factor 2
NCBI Official Synonym Full Names
fibroblast growth factor 2 (basic)
NCBI Official Symbol
NCBI Official Synonym Symbols
FGF-2
  [Similar Products]
NCBI Protein Information
fibroblast growth factor 2; BFGF; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2
UniProt Protein Name
Fibroblast growth factor 2
UniProt Synonym Protein Names
Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
FGF-2; bFGF; HBGF-2  [Similar Products]
UniProt Entry Name
FGF2_BOVIN
UniProt Comments for FGF2
Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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