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FGF2 elisa kit :: Human FGF2 ELISA Kit

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Catalog # MBS8244557
Unit / Price
  96 Tests  /  $470 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

FGF2, ELISA Kit

Popular Item
Full Product Name

Human FGF2 ELISA Kit

Product Synonym Names
FGFB; Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
134920
3D Structure
ModBase 3D Structure for P09038
Species Reactivity
Specificity
The Human FGF2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human FGF2 proteins within the range of 7.8 pg/ml - 500 pg/ml.
Sensitivity
<4 pg/mL
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FGF2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFGF2purchase
MBS8244557 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FGF2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FGF2. The ELISA analytical biochemical technique of the MBS8244557 kit is based on FGF2 antibody-FGF2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FGF2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FGF2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FGF2 elisa kit
Principle of the Assay: MBS Human FGF2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF2 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human FGF2 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Human FGF-Basic or Fibroblast Growth Factor 2, also known commonly as HBGF-2 or Heparin-Binding Growth Factor 2, is a 288 amino acid cytokine protein encoded by the FGF2gene at locus 4q26 on chromosome 4. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, would healing and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. The monomeric FGF-Basic protein interacts with many other proteins such as CSPG4 and FGFBP1 while also believed to be found in a complex with FGFBP1, FGF1 and FGF2. Expression patterns for this protein typically take place in granulosa and cumulus cells, but FGF2 is also found in hepatocellular carcinoma cells (not non-cancerous liver tissue). Several experiments were performed on FGF-Basic to determine its pH and temperature properties. It has been recently determined that FGF-Basic retains almost half of its activity after treatment at pH 2.0 for 3 hours at 20°C, while being inactivated after 3 minutes at 60°C or 1 minute at 80°C.
Applications Tested/Suitable for FGF2 elisa kit
Sandwich ELISA (EIA)

Typical Testing Data/Standard Curve (for reference only) of FGF2 elisa kit
FGF2 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS8244557. Click to request current manual
NCBI/Uniprot data below describe general gene information for FGF2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,203 Da
NCBI Official Full Name
Fibroblast growth factor 2
NCBI Official Synonym Full Names
fibroblast growth factor 2
NCBI Official Symbol
NCBI Official Synonym Symbols
BFGF; FGFB; FGF-2; HBGF-2
  [Similar Products]
NCBI Protein Information
fibroblast growth factor 2
UniProt Protein Name
Fibroblast growth factor 2
UniProt Synonym Protein Names
Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
FGFB; FGF-2; bFGF; HBGF-2  [Similar Products]
UniProt Entry Name
FGF2_HUMAN
NCBI Summary for FGF2
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
UniProt Comments for FGF2
FGF2: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. Belongs to the heparin-binding growth factors family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Activator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q26

Cellular Component: extracellular region; extracellular space; nucleus

Molecular Function: chemoattractant activity; cytokine activity; fibroblast growth factor receptor binding; growth factor activity; heparin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding

Biological Process: activation of MAPK activity; activation of MAPKK activity; axon guidance; cell migration during sprouting angiogenesis; chemotaxis; embryonic morphogenesis; epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; fibroblast growth factor receptor signaling pathway; growth factor dependent regulation of satellite cell proliferation; hyaluronan catabolic process; innate immune response; inositol phosphate biosynthetic process; insulin receptor signaling pathway; MAPKKK cascade; negative regulation of blood vessel endothelial cell migration; nerve growth factor receptor signaling pathway; nervous system development; organ morphogenesis; phosphatidylinositol biosynthetic process; phosphoinositide-mediated signaling; positive chemotaxis; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cardiac muscle cell proliferation; positive regulation of cell division; positive regulation of cell fate specification; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of MAP kinase activity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; Ras protein signal transduction; regulation of angiogenesis; release of sequestered calcium ion into cytosol; signal transduction; small GTPase mediated signal transduction; somatic stem cell maintenance; ureteric bud branching; vascular endothelial growth factor receptor signaling pathway; wound healing
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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