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FGF9 elisa kit :: Human FGF9 ELISA Kit

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Catalog # MBS176006
Unit / Price
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  96-Strip-Wells  /  $485 +1 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

FGF9, ELISA Kit

Popular Item
Full Product Name

Human FGF9 PicoKine ELISA Kit

Product Synonym Names
Fibroblast growth factor 9; fibroblast growth factor 9; Elbow knee synostosis; FGF 9; FGF-9; FGF9; FGF9_HUMAN; Fibroblast growth factor 9; GAF; Glia Activating Factor; Glia-activating factor; HBFG 9; HBFG9; HBGF-9; Heparin binding growth factor 9; Heparin-binding growth factor 9; MGC119914; MGC119915; SYNS3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
gene 612961
3D Structure
ModBase 3D Structure for P31371
Species Reactivity
Specificity
Natural and recombinant human FGF9
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 15pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FGF9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for FGF9purchase
MBS176006 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FGF9, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FGF9. The ELISA analytical biochemical technique of the MBS176006 kit is based on FGF9 antibody-FGF9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FGF9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FGF9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FGF9 elisa kit
Principle of the assay: human FGF9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF9 has been precoated onto 96-well plates. Standards(sf21,M1-S208) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF9 amount of sample captured in plate.
Background: Fibroblast growth factor-9 (FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells.1 The human FGF-9 cDNA cloned by using oligonucleotide probes encodes a polypeptide consisting of 208amino acids. Sequence similarity to other members of the FGF family has been estimated to be around 30%.2FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma.3 FGF9 is produced and secreted by the prostatic stromal cells. It is a potent mitogen for bothprostatic epithelial and stromal cells in culture. FGF9 is an abundant secreted growth factor that can act as both a paracrine mitogen for epithelial cells and an autocrine mitogen for stromal cells. Overexpression of this paracrine and autocrine growth factor may play an important role in the epithelial and stromal proliferation in benign prostatic hyperplasia.4 As a result of glycosylation, the molecular mass is 25-27KDa.

Typical Testing Data/Standard Curve (for reference only) of FGF9 elisa kit
FGF9 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS176006. Click to request current manual
NCBI/Uniprot data below describe general gene information for FGF9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,441 Da
NCBI Official Full Name
Fibroblast growth factor 9
NCBI Official Synonym Full Names
fibroblast growth factor 9
NCBI Official Symbol
NCBI Official Synonym Symbols
GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
  [Similar Products]
NCBI Protein Information
fibroblast growth factor 9; heparin-binding growth factor 9; fibroblast growth factor 9 (glia-activating factor)
UniProt Protein Name
Fibroblast growth factor 9
UniProt Synonym Protein Names
Glia-activating factor; GAF; Heparin-binding growth factor 9; HBGF-9
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
FGF-9; GAF; HBGF-9  [Similar Products]
UniProt Entry Name
FGF9_HUMAN
NCBI Summary for FGF9
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq, Jul 2008]
UniProt Comments for FGF9
FGF9: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3). Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 13q11-q12

Cellular Component: extracellular space; cytoplasm; extracellular region; basement membrane

Molecular Function: heparin binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; embryonic skeletal development; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of activin receptor signaling pathway; substantia nigra development; cell-cell signaling; positive regulation of MAPKKK cascade; protein import into nucleus; male sex determination; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; chondrocyte differentiation; positive regulation of smoothened signaling pathway; angiogenesis; regulation of timing of cell differentiation; embryonic gut development; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; inner ear morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; male gonad development; osteoblast differentiation; positive regulation of cell division; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation

Disease: Multiple Synostoses Syndrome 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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