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anti-FLT3 antibody :: anti-Human, Mouse FLT3 Antibody

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Catalog # MBS851371
Unit / Price
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  0.1 mg  /  $215 +1 FREE 8GB USB
Western Blot (WB)
Product Name

FLT3, Antibody

Popular Item
Full Product Name

FLT3 (Phospho-Tyr842) Antibody

Product Synonym Names
CD135; EC 2.7.10.1; fetal liver kinase 2; FL cytokine receptor; FLK-2; FLT-3; FLT3; fms-related tyrosine kinase 3; kinase Flt3; stem cell tyrosine kinase 1; STK-1; STK1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
136351
3D Structure
ModBase 3D Structure for P36888
Species Reactivity
Human, Mouse
Specificity
FLT3 (Phospho-Tyr842) antibody detects endogenous levels of FLT3 only when phosphorylated at tyrosine 842.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human FLT3 around the phosphorylation site of tyrosine 842 (S-N-YP-V-V).
All Sites
Human: Tyr842
Mouse: Tyr845
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-FLT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-FLT3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-FLT3 antibody
WB: 1:500-1:1000
ELISA: 1:10000

Western Blot (WB) of anti-FLT3 antibody
Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using FLT3 (Phospho-Tyr842)) antibody.
anti-FLT3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for FLT3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
108,378 Da
NCBI Official Full Name
receptor-type tyrosine-protein kinase FLT3
NCBI Official Synonym Full Names
fms related tyrosine kinase 3
NCBI Official Symbol
NCBI Official Synonym Symbols
FLK2; STK1; CD135; FLK-2
  [Similar Products]
NCBI Protein Information
receptor-type tyrosine-protein kinase FLT3
UniProt Protein Name
Receptor-type tyrosine-protein kinase FLT3
UniProt Synonym Protein Names
FL cytokine receptor; Fetal liver kinase-2; FLK-2; Fms-like tyrosine kinase 3; FLT-3; Stem cell tyrosine kinase 1; STK-1; CD_antigen: CD135
UniProt Gene Name
UniProt Synonym Gene Names
CD135; FLK2; STK1; FLK-2; FLT-3; STK-1  [Similar Products]
NCBI Summary for FLT3
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015]
UniProt Comments for FLT3
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
Product References and Citations for anti-FLT3 antibody
Small D., Proc. Natl. Acad. Sci. U.S.A. 91:459-463(1994). Rosnet O., Blood 82:1110-1119(1993). Rosnet O., Genomics 9:380-385(1991).

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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