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Flt3L elisa kit :: Mouse Flt3L ELISA Kit

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Catalog # MBS2505326
Unit / Price
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  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Flt3L, ELISA Kit

Popular Item
Full Product Name

Mouse Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
600007
3D Structure
ModBase 3D Structure for P49771
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse Flt3L. No significant cross-reactivity or interference between Mouse Flt3L and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse Flt3L and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Flt3L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Flt3Lpurchase
MBS2505326 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Flt3L, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Flt3L. The ELISA analytical biochemical technique of the MBS2505326 kit is based on Flt3L antibody-Flt3L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Flt3L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Flt3L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Flt3L elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Flt3L in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Flt3L. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse Flt3L and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Flt3L, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse Flt3L. You can calculate the concentration of Mouse Flt3L in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of Flt3L elisa kit
Flt3L elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2505326. Click to request current manual
NCBI/Uniprot data below describe general gene information for Flt3L. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,290 Da
NCBI Official Full Name
fms-related tyrosine kinase 3 ligand isoform 2
NCBI Official Synonym Full Names
fms-related tyrosine kinase 3 ligand
NCBI Official Symbol
FLT3LG  [Similar Products]
NCBI Official Synonym Symbols
FL; FLT3L
  [Similar Products]
NCBI Protein Information
fms-related tyrosine kinase 3 ligand; flt3 ligand
UniProt Protein Name
Fms-related tyrosine kinase 3 ligand
UniProt Synonym Protein Names
SL cytokine
UniProt Gene Name
FLT3LG  [Similar Products]
UniProt Synonym Gene Names
Flt3 ligand; Flt3L  [Similar Products]
UniProt Entry Name
FLT3L_HUMAN
NCBI Summary for Flt3L
Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]
UniProt Comments for Flt3L
FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding; receptor binding

Biological Process: regulation of myeloid dendritic cell activation; positive regulation of natural killer cell differentiation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; signal transduction; positive regulation of myoblast differentiation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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