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FN elisa kit :: Rat FN ELISA Kit

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Catalog # MBS2509415
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

FN, ELISA Kit

Popular Item
Also Known As

Rat FN (Fibronectin) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
2477
OMIM
135600
3D Structure
ModBase 3D Structure for P02751
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat FN. No significant cross-reactivity or interference between Rat FN and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat FN and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.25-80ng/mL
Sensitivity
Min: 0.75ng/mL; Max: 80ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFNpurchase
MBS2509415 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FN, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FN. The ELISA analytical biochemical technique of the MBS2509415 kit is based on FN antibody-FN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FN elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FN in Rat serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FN. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat FN and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat FN. You can calculate the concentration of Rat FN in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of FN elisa kit
FN elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2509415. Click to request current manual
NCBI/Uniprot data below describe general gene information for FN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
256,502 Da
NCBI Official Full Name
fibronectin isoform 1 preproprotein
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
NCBI Official Synonym Symbols
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
  [Similar Products]
NCBI Protein Information
fibronectin; cold-insoluble globulin; migration-stimulating factor
UniProt Protein Name
Fibronectin
UniProt Synonym Protein Names
Cold-insoluble globulin; CIG
UniProt Gene Name
UniProt Synonym Gene Names
FN; FN; CIG  [Similar Products]
UniProt Entry Name
FINC_HUMAN
NCBI Summary for FN
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for FN
FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: extracellular matrix; extracellular space; fibrinogen complex; apical plasma membrane; ER-Golgi intermediate compartment; extracellular region; basal lamina

Molecular Function: integrin binding; heparin binding; collagen binding; protein binding; protease activator activity; protease binding

Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; regulation of cell shape; extracellular matrix disassembly; platelet degranulation; acute-phase response; cell-substrate junction assembly; response to wounding; calcium-independent cell-matrix adhesion; angiogenesis; peptide cross-linking; blood coagulation; cell adhesion; leukocyte migration

Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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