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FOS elisa kit :: Goat V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog ELISA Kit

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Catalog # MBS734147
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog, ELISA Kit

Also Known As

Goat V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 14; NC_000014.8 (75745481..75748937). Location: 14q24.3
3D Structure
ModBase 3D Structure for Q6FG41
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This FOS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat FOS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FOS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFOSpurchase
MBS734147 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FOS. The ELISA analytical biochemical technique of the MBS734147 kit is based on FOS antibody-FOS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FOS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FOS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FOS elisa kit
Principle of the Assay: FOS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FOS antibody and an FOS-HRP conjugate. The assay sample and buffer are incubated together with FOS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FOS concentration since FOS from samples and FOS-HRP conjugate compete for the anti-FOS antibody binding site. Since the number of sites is limited, as more sites are occupied by FOS from the sample, fewer sites are left to bind FOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FOS concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FOS elisa kit

Typical Testing Data/Standard Curve (for reference only) of FOS elisa kit
FOS elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS734147. Click to request current manual
NCBI/Uniprot data below describe general gene information for FOS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
40,695 Da
NCBI Official Full Name
FOS
NCBI Official Synonym Full Names
FBJ murine osteosarcoma viral oncogene homolog
NCBI Official Symbol
NCBI Official Synonym Symbols
p55; AP-1; C-FOS
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NCBI Protein Information
proto-oncogene c-Fos; activator protein 1; cellular oncogene c-fos; G0/G1 switch regulatory protein 7; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)
UniProt Protein Name
FOS protein
Protein Family
UniProt Gene Name
UniProt Entry Name
Q6FG41_HUMAN
NCBI Summary for FOS
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
UniProt Comments for FOS
Sequence similarities: Contains 1 bZIP domain. SAAS SAAS000837
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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