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FOS elisa kit :: Rat V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit

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Catalog # MBS2020036 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
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Typical Testing Data/Standard Curve (for reference only)
Product Name

V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS), ELISA Kit

Popular Item
Full Product Name

V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit

Product Synonym Names
Cfos; C-Fos; G0S7; Proto-oncogene c-Fos; G0/G1 Switch Regulatory Protein 7; Cellular oncogene fos
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
164810
3D Structure
ModBase 3D Structure for P01100
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS).
No significant cross-reactivity or interference between V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.060ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FOS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for FOSpurchase
MBS2020036 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FOS. The ELISA analytical biochemical technique of the MBS2020036 kit is based on FOS antibody-FOS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FOS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FOS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FOS elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for FOS elisa kit

Typical Testing Data/Standard Curve (for reference only) of FOS elisa kit
FOS elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2020036. Click to request current manual
NCBI/Uniprot data below describe general gene information for FOS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36,301 Da
NCBI Official Full Name
V-fos FBJ murine osteosarcoma viral oncogene homolog
NCBI Official Synonym Full Names
FBJ murine osteosarcoma viral oncogene homolog
NCBI Official Symbol
NCBI Official Synonym Symbols
p55; AP-1; C-FOS
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NCBI Protein Information
proto-oncogene c-Fos
UniProt Protein Name
Proto-oncogene c-Fos
UniProt Synonym Protein Names
Cellular oncogene fos; G0/G1 switch regulatory protein 7
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
FOS_HUMAN
NCBI Summary for FOS
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
UniProt Comments for FOS
Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.

Protein type: Oncoprotein; Transcription factor; Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; nucleus; cytosol

Molecular Function: protein binding; double-stranded DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to gravity; response to cAMP; positive regulation of osteoclast differentiation; response to toxin; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; response to lipopolysaccharide; female pregnancy; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; conditioned taste aversion; transforming growth factor beta receptor signaling pathway; DNA methylation; inflammatory response; toll-like receptor 4 signaling pathway; response to corticosterone stimulus; aging; response to drug; nervous system development; response to light stimulus; MyD88-independent toll-like receptor signaling pathway; sleep; cellular response to hormone stimulus; toll-like receptor 2 signaling pathway; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; response to mechanical stimulus; response to cytokine stimulus; toll-like receptor signaling pathway; cellular response to extracellular stimulus; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to cold; toll-like receptor 9 signaling pathway; response to progesterone stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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