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FOXO1 elisa kit :: Human Forkhead Box Protein O1 ELISA Kit

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Catalog # MBS023752
Unit / Price
  48-Strip-Wells  /  $470 +1 FREE 8GB USB
  96-Strip-Wells  /  $680 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,100 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $6,095 +7 FREE 8GB USB
FOXO1 elisa kit
Product Name

Forkhead Box Protein O1 (FOXO1), ELISA Kit

Popular Item
Also Known As

Human Forkhead Box Protein O1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 13; NC_000013.10 (41129801..41240734, complement). Location: 13q14.1
OMIM
136533
3D Structure
ModBase 3D Structure for Q12778
Species Reactivity
Specificity
No significant cross-reactivity or interference between Human FOXO1 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
31.2 pg/ml - 1000 pg/ml.
Sensitivity
5.0 pg/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FOXO1 concentrations in Human serum, plasma and other body fluids. Using Purified Human FOXO1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FOXO1 and FOXO1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FOXO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FOXO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFOXO1purchase
MBS023752 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Forkhead Box Protein O1 (FOXO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FOXO1. The ELISA analytical biochemical technique of the MBS023752 kit is based on FOXO1 antibody-FOXO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FOXO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FOXO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FOXO1 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FOXO1 concentrations in Human serum, plasma and other body fluids. Using Purified Human FOXO1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FOXO1 and FOXO1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FOXO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS023752. Click to request current manual
NCBI/Uniprot data below describe general gene information for FOXO1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
69,662 Da
NCBI Official Full Name
forkhead box protein O1
NCBI Official Synonym Full Names
forkhead box O1
NCBI Official Symbol
FOXO1  [Similar Products]
NCBI Official Synonym Symbols
FKH1; FKHR; FOXO1A
  [Similar Products]
NCBI Protein Information
forkhead box protein O1; forkhead box protein O1A; forkhead, Drosophila, homolog of, in rhabdomyosarcoma
UniProt Protein Name
Forkhead box protein O1
UniProt Synonym Protein Names
Forkhead box protein O1A; Forkhead in rhabdomyosarcoma
Protein Family
UniProt Gene Name
FOXO1  [Similar Products]
UniProt Synonym Gene Names
FKHR; FOXO1A  [Similar Products]
UniProt Entry Name
FOXO1_HUMAN
NCBI Summary for FOXO1
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]
UniProt Comments for FOXO1
FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.

Protein type: Oncoprotein; Transcription factor; DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding; chromatin binding

Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; thermoregulation; positive regulation of gluconeogenesis; positive regulation of autophagy; epidermal growth factor receptor signaling pathway; blood vessel development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; negative regulation of stress-activated MAPK cascade; cellular response to starvation; negative regulation of fat cell differentiation; endocrine pancreas development; protein amino acid acetylation; cellular response to insulin stimulus; positive regulation of protein catabolic process; insulin receptor signaling pathway; autophagy; innate immune response; cell glucose homeostasis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; negative regulation of apoptosis

Disease: Rhabdomyosarcoma 2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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