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anti-FOXO3 antibody :: Rabbit FOXO3-S253 Antibody

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Catalog # MBS128272
Unit / Price
  0.05 mL  /  $265 +1 FREE 8GB USB
  0.1 mL  /  $380 +1 FREE 8GB USB
  0.2 mL  /  $555 +1 FREE 8GB USB
Western Blot (WB)
Product Name

FOXO3-S253, Antibody

Popular Item
Also Known As

Phospho-FOXO3-S253 Polyclonal Antibody

Product Synonym Names
Phospho-FOXO3-S253: FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
673
OMIM
602681
3D Structure
ModBase 3D Structure for O43524
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Route
Synthetic Peptide
Category
Primary antibody
Immunogen
A phospho specific peptide corresponding to residues surrounding S253 of human FOXO3
Calculated MW
48kDa/71kDa
Observed MW
100kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-FOXO3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-FOXO3 antibody
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Product Categories/Family for anti-FOXO3 antibody
Applications Tested/Suitable for anti-FOXO3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-FOXO3 antibody
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:50 - 1:200

Western Blot (WB) of anti-FOXO3 antibody
Western blot analysis of extracts of Untreated and treated 293 cell line, using Phospho-FOXO3-S253 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
anti-FOXO3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for FOXO3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Forkhead box protein O3
NCBI Official Synonym Full Names
forkhead box O3
NCBI Official Symbol
FOXO3  [Similar Products]
NCBI Official Synonym Symbols
FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
  [Similar Products]
NCBI Protein Information
forkhead box protein O3; forkhead box O3A; forkhead in rhabdomyosarcoma-like 1; forkhead homolog (rhabdomyosarcoma) like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
UniProt Protein Name
Forkhead box protein O3
UniProt Synonym Protein Names
AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1
Protein Family
UniProt Gene Name
FOXO3  [Similar Products]
UniProt Synonym Gene Names
FKHRL1; FOXO3A  [Similar Products]
UniProt Entry Name
FOXO3_HUMAN
NCBI Summary for FOXO3
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
UniProt Comments for FOXO3
FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.

Protein type: Oncoprotein; DNA-binding; Transcription factor; Autophagy

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; DNA binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator; nerve growth factor receptor signaling pathway; initiation of primordial ovarian follicle growth; positive regulation of transcription, DNA-dependent; antral ovarian follicle growth; positive regulation of erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; ovulation from ovarian follicle; oocyte maturation; regulation of translation; regulation of transcription from RNA polymerase II promoter; positive regulation of neuron apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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