• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

FRZB3 elisa kit :: Human Secreted frizzled related protein 3 (FRZB) ELISA Kit

Scan QR to view Datasheet
Catalog # MBS7238827
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Secreted frizzled related protein 3 (FRZB) (FRZB3), ELISA Kit

Popular Item
Also Known As

Human Secreted frizzled related protein 3 (FRZB) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605083
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of FRZB. No significant cross-reactivity or interference between FRZB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FRZB and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FRZB3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFRZB3purchase
MBS7238827 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Secreted frizzled related protein 3 (FRZB) (FRZB3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FRZB3. The ELISA analytical biochemical technique of the MBS7238827 kit is based on FRZB3 antibody-FRZB3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FRZB3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FRZB3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FRZB3 elisa kit
Intended Uses: This FRZB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FRZB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: FRZB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FRZB antibody and an FRZB-HRP conjugate. The assay sample and buffer are incubated together with FRZB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FRZB concentration since FRZB from samples and FRZB-HRP conjugate compete for the anti-FRZB antibody binding site. Since the number of sites is limited, as more sites are occupied by FRZB from the sample, fewer sites are left to bind FRZB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FRZB concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FRZB3 elisa kit

Typical Standard Curve/Testing Data of FRZB3 elisa kit
FRZB3 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS7238827. Click to request current manual
NCBI/Uniprot data below describe general gene information for FRZB3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
36,254 Da
NCBI Official Full Name
secreted frizzled-related protein 3
NCBI Official Synonym Full Names
frizzled-related protein
NCBI Official Symbol
NCBI Official Synonym Symbols
FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN
  [Similar Products]
NCBI Protein Information
secreted frizzled-related protein 3; sFRP-3; frezzled; frizzled homolog-related; frizzled-related protein 1
UniProt Protein Name
Secreted frizzled-related protein 3
UniProt Synonym Protein Names
Frezzled; Fritz; Frizzled-related protein 1; FrzB-1
UniProt Gene Name
UniProt Synonym Gene Names
FIZ; FRE; FRP; FRZB1; SFRP3; sFRP-3  [Similar Products]
UniProt Entry Name
SFRP3_HUMAN
NCBI Summary for FRZB3
The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. [provided by RefSeq, Apr 2010]
UniProt Comments for FRZB3
FRZB: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development. Defects in FRZB are associated with susceptibility to osteoarthritis type 1(OS1). Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity

Biological Process: negative regulation of cell proliferation; convergent extension involved in organogenesis; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; positive regulation of fat cell differentiation; neural crest cell differentiation; negative regulation of cell growth; skeletal development

Disease: Osteoarthritis Susceptibility 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000