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FSH elisa kit :: Rat follicle-stimulating hormone, FSH ELISA Kit

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Catalog # MBS700927
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,615 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,940 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

follicle-stimulating hormone, FSH, ELISA Kit

Popular Item
Also Known As

rat follicle-stimulating hormone, FSH ELISA Kit

Product Synonym Names
rat follicle-stimulating hormone; FSH ELISA Kit; follicle-stimulating hormone; FSH
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS700927 Testing Data
Sequence Length
801
OMIM
601540
3D Structure
ModBase 3D Structure for P25440
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of rat FSH. No significant cross-reactivity or interference between rat FSH and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Detection Range
0.25 mlU/ml-100 mlU/ml
Sensitivity
0.25 mlU/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FSH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFSHpurchase
MBS700927 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the follicle-stimulating hormone, FSH, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FSH. The ELISA analytical biochemical technique of the MBS700927 kit is based on FSH antibody-FSH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FSH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FSH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FSH elisa kit
Introduction: Follicle-stimulating hormone (FSH) is a hormone synthesized and secreted by gonadotropes in the anterior pituitary gland. FSH regulates the development, growth, pubertal maturation, and reproductive processes of the human body. FSH and Luteinizing hormone (LH) act synergistically in reproduction. FSH is a glycoprotein. Each monomeric unit is a protein molecule with a sugar attached to it; two of these make the full, functional protein. Its structure is similar to those of LH, TSH, and hCG. The protein dimer contains 2 polypeptide units, labeled alpha and beta subunits. The alpha subunits of LH, FSH, TSH, and hCG are identical, and contain 92 amino acids. The beta subunits vary. FSH has a beta subunit of 118 amino acids (FSHB), which confers its specific biologic action and is responsible for interaction with the FSH-receptor. The sugar part of the hormone is composed of fucose, galactose, mannose, galactosamine, glucosamine, and sialic acid, the latter being critical for its biologic half-life. The half-life of FSH is 3-4 hours. Its molecular wt is 30000.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with a goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated FSH and antibody preparation specific for FSH and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of FSH elisa kit
FSH elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS700927. Click to request current manual
NCBI/Uniprot data below describe general gene information for FSH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
74,881 Da
NCBI Official Full Name
FSH
UniProt Protein Name
Bromodomain-containing protein 2
UniProt Synonym Protein Names
O27.1.1; Really interesting new gene 3 protein
UniProt Gene Name
UniProt Synonym Gene Names
KIAA9001; RING3  [Similar Products]
UniProt Entry Name
BRD2_HUMAN
UniProt Comments for FSH
Function: May play a role in spermatogenesis or folliculogenesis Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly.1 PublicationManual assertion based on experiment in:Ref.8
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withFSH elisa kit
 Reference Product  PubMed Publications
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