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FSTL3 elisa kit :: Mouse Follistatin Like Protein 3 (FSTL3) ELISA Kit

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Catalog # MBS2021344 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Follistatin Like Protein 3 (FSTL3), ELISA Kit

Popular Item
Full Product Name

Follistatin Like Protein 3 (FSTL3) ELISA Kit

Product Synonym Names
FLRG; FSRP; Follistatin-Related Protein,Secreted Glycoprotein; Follistatin-related gene protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
605343
3D Structure
ModBase 3D Structure for O95633
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Follistatin Like Protein 3 (FSTL3).
No significant cross-reactivity or interference between Follistatin Like Protein 3 (FSTL3) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
15.6-1000pg/mL
Sensitivity
< 6.4pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Follistatin Like Protein 3 (FSTL3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Follistatin Like Protein 3 (FSTL3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FSTL3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for FSTL3purchase
MBS2021344 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Follistatin Like Protein 3 (FSTL3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FSTL3. The ELISA analytical biochemical technique of the MBS2021344 kit is based on FSTL3 antibody-FSTL3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FSTL3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FSTL3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FSTL3 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follistatin Like Protein 3 (FSTL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Follistatin Like Protein 3 (FSTL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Follistatin Like Protein 3 (FSTL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Follistatin Like Protein 3 (FSTL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for FSTL3 elisa kit

Typical Testing Data/Standard Curve (for reference only) of FSTL3 elisa kit
FSTL3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2021344. Click to request current manual
NCBI/Uniprot data below describe general gene information for FSTL3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,960 Da
NCBI Official Full Name
follistatin-related protein 3
NCBI Official Synonym Full Names
follistatin-like 3 (secreted glycoprotein)
NCBI Official Symbol
FSTL3  [Similar Products]
NCBI Official Synonym Symbols
FLRG; FSRP
  [Similar Products]
NCBI Protein Information
follistatin-related protein 3
UniProt Protein Name
Follistatin-related protein 3
UniProt Synonym Protein Names
Follistatin-like protein 3; Follistatin-related gene protein
UniProt Gene Name
FSTL3  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
FSTL3_HUMAN
NCBI Summary for FSTL3
Follistatin-like 3 is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis. [provided by RefSeq, Jul 2008]
UniProt Comments for FSTL3
FSTL3: Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Secreted; Oncoprotein; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: nucleoplasm; Golgi apparatus; extracellular space; extracellular region; nucleus; secretory granule

Molecular Function: protein binding; fibronectin binding; activin binding

Biological Process: negative regulation of osteoclast differentiation; ossification; transcription, DNA-dependent; adrenal gland development; male gonad development; negative regulation of BMP signaling pathway; regulation of transcription from RNA polymerase II promoter; negative regulation of activin receptor signaling pathway; positive regulation of cell-cell adhesion; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; spermatogenesis; kidney development; lung development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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