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FTO elisa kit :: Human Alpha-ketoglutarate-dependent dioxygenase FTO ELISA Kit

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Catalog # MBS2887853
Unit / Price
  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Alpha-ketoglutarate-dependent dioxygenase FTO, ELISA Kit

Also Known As

Human Alpha-ketoglutarate-dependent dioxygenase FTO ELISA Kit

Product Synonym Names
Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; FTO; KIAA1752; 1.14.11.-
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
505
OMIM
601665
Species Reactivity
Specificity
This assay recognizes recombinant and natural human Alpha-ketoglutarate-dependent dioxygenase FTO. No significant cross-reactivity or interference was observed.
Samples
Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
0.31 ng/mL -20.0 ng/mL.
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FTO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFTOpurchase
MBS2887853 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-ketoglutarate-dependent dioxygenase FTO, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FTO. The ELISA analytical biochemical technique of the MBS2887853 kit is based on FTO antibody-FTO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FTO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FTO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FTO elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Alpha-ketoglutarate-dependent dioxygenase FTO concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-ketoglutarate-dependent dioxygenase FTO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-ketoglutarate-dependent dioxygenase FTO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-ketoglutarate-dependent dioxygenase FTO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-ketoglutarate-dependent dioxygenase FTO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of FTO elisa kit
FTO elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2887853. Click to request current manual
NCBI/Uniprot data below describe general gene information for FTO. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
12,218 Da
NCBI Official Full Name
alpha-ketoglutarate-dependent dioxygenase FTO
NCBI Official Synonym Full Names
fat mass and obesity associated
NCBI Official Symbol
NCBI Official Synonym Symbols
GDFD; ALKBH9; BMIQ14
  [Similar Products]
NCBI Protein Information
alpha-ketoglutarate-dependent dioxygenase FTO
UniProt Protein Name
Alpha-ketoglutarate-dependent dioxygenase FTO
UniProt Synonym Protein Names
Fat mass and obesity-associated protein
UniProt Gene Name
UniProt Synonym Gene Names
KIAA1752  [Similar Products]
UniProt Entry Name
FTO_HUMAN
NCBI Summary for FTO
This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]
UniProt Comments for FTO
FTO: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD). A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years. Belongs to the fto family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: nuclear speck; nucleus

Molecular Function: DNA-N1-methyladenine dioxygenase activity; ferrous iron binding

Biological Process: DNA dealkylation; RNA repair

Disease: Body Mass Index Quantitative Trait Locus 14; Growth Retardation, Developmental Delay, Coarse Facies, And Early Death
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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