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FUCa1 elisa kit :: Human Fucosidase, Alpha L1, Tissue ELISA Kit

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Catalog # MBS732061
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Fucosidase, Alpha L1, Tissue (FUCa1), ELISA Kit

Popular Item
Also Known As

Human Fucosidase, Alpha L1, Tissue ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
612280
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of AFU. No significant cross-reactivity or interference between AFU and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AFU and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This AFU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AFU. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FUCa1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFUCa1purchase
MBS732061 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fucosidase, Alpha L1, Tissue (FUCa1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FUCa1. The ELISA analytical biochemical technique of the MBS732061 kit is based on FUCa1 antibody-FUCa1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FUCa1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FUCa1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FUCa1 elisa kit
Principle of the assay: AFU ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AFU. Standards or samples are then added to the microtiter plate wells and AFU if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AFU present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AFU are added to each well to “sandwich” the AFU immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AFU and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFU concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FUCa1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of FUCa1 elisa kit
FUCa1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS732061. Click to request current manual
NCBI/Uniprot data below describe general gene information for FUCa1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,689 Da
NCBI Official Full Name
fucosidase, alpha-L- 1, tissue
NCBI Official Synonym Full Names
fucosidase, alpha-L- 1, tissue
NCBI Official Symbol
FUCA1  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
tissue alpha-L-fucosidase; alpha-L-fucosidase 1; alpha-L-fucosidase I; alpha-L-fucoside fucohydrolase 1
UniProt Protein Name
Tissue alpha-L-fucosidase
UniProt Synonym Protein Names
Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; Alpha-L-fucosidase 1
Protein Family
UniProt Gene Name
FUCA1  [Similar Products]
UniProt Synonym Gene Names
Nbla10230; Alpha-L-fucosidase 1  [Similar Products]
UniProt Entry Name
FUCO_HUMAN
NCBI Summary for FUCa1
The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]
UniProt Comments for FUCa1
FUCA1: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins. Defects in FUCA1 are the cause of fucosidosis (FUCA1D). FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. Belongs to the glycosyl hydrolase 29 family.

Protein type: Hydrolase; EC 3.2.1.51; Glycan Metabolism - other glycan degradation

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: lysosome; cytoplasm

Molecular Function: alpha-L-fucosidase activity; fucose binding

Biological Process: fucose metabolic process; glycosaminoglycan catabolic process; glycoside catabolic process

Disease: Fucosidosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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