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FVII elisa kit :: Mouse Coagulation Factor VII ELISA Kit

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Catalog # MBS760667
Unit / Price
  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Coagulation Factor VII (FVII), ELISA Kit

Popular Item
Also Known As

Mouse Coagulation Factor VII ELISA Kit

Product Synonym Names
FVII/F7/Proconvertin/SPCA
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS760667 COA
Sequence Length
21
OMIM
227500
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of FVII . No significant cross-reactivity or interference between FVII and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/ml
Sensitivity
<0.094ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FVII were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FVII were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of FVII elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forFVIIpurchase
MBS760667 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Coagulation Factor VII (FVII) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FVII. The ELISA analytical biochemical technique of the MBS760667 kit is based on FVII antibody-FVII antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FVII antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FVII. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
FVII elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FVII antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- FVII antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FVII amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FVII can be calculated.

Typical Testing Data/Standard Curve (for reference only) of FVII elisa kit
FVII elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS760667. Click to request current manual
NCBI/Uniprot data below describe general gene information for FVII. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,320 Da
NCBI Official Full Name
coagulation factor VII, partial
NCBI Official Synonym Full Names
coagulation factor VII
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
coagulation factor VII
UniProt Protein Name
Coagulation factor VII
UniProt Synonym Protein Names
Proconvertin; Serum prothrombin conversion accelerator; SPCA
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
FA7_HUMAN
NCBI Summary for FVII
This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]
UniProt Comments for FVII
F7: Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. Defects in F7 are the cause of factor VII deficiency (FA7D). A hemorrhagic disease with variable presentation. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or repeated hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Finally, numerous subjects are completely asymptomatic despite very low factor VII levels. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Motility/polarity/chemotaxis; Secreted; EC 3.4.21.21; Secreted, signal peptide; Apoptosis

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; vesicle

Molecular Function: calcium ion binding; glycoprotein binding; protein binding; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: blood coagulation; blood coagulation, extrinsic pathway; cellular protein metabolic process; circadian rhythm; ER to Golgi vesicle-mediated transport; organ regeneration; peptidyl-glutamic acid carboxylation; positive regulation of blood coagulation; positive regulation of cell migration; positive regulation of leukocyte chemotaxis; positive regulation of positive chemotaxis; positive regulation of protein kinase B signaling cascade; post-translational protein modification; response to estrogen stimulus; response to vitamin K; signal peptide processing

Disease: Factor Vii Deficiency; Myocardial Infarction, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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