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G6PD recombinant protein

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Catalog # MBS203264
Unit / Price
  0.1 mg  /  $255 +1 FREE 8GB USB
  0.5 mg  /  $535 +1 FREE 8GB USB
SDS-Page
Product Name

G6PD, Recombinant Protein

Popular Item
Also Known As

G6PD,1-491aa, E Coli, E Coli (Bioactivity Validated)

Product Synonym Names
Glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase; zwf; Glucose-6-phosphate 1-dehydrogenase G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; MET19; POS10; Zwf1p.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
491
Sequence
MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
3D Structure
ModBase 3D Structure for P0AC53
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
In 50 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
E Coli
Biological Activity
Specific activity is > 70 units/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of G6PD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
G6PD recombinant protein
Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E Coli and purified by conventional chromatography techniques.
Product Categories/Family for G6PD recombinant protein
Applications Tested/Suitable for G6PD recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for G6PD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
55.7kDa (491 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
glucose-6-phosphate 1-dehydrogenase
NCBI Official Symbol
NCBI Official Synonym Symbols
ECK1853; JW1841
  [Similar Products]
NCBI Protein Information
glucose-6-phosphate 1-dehydrogenase
UniProt Protein Name
Glucose-6-phosphate 1-dehydrogenase
UniProt Gene Name
UniProt Synonym Gene Names
NCBI Summary for G6PD
Residues 199-203 of zwf encode a NNWDN peptide involved in ChpA(MazF) toxicity that is likely converted to NNWNN by AsnA; a zwf deletion prevents NNWNN activity (Kolodkin-Gal, 2007). [More information is available at EcoGene: EG11221]. A null mutation in the zwf gene encoding glucose 6-phosphate-1-dehydrogenase does not affect the growth rate significantly. [More information is available at EcoCyc: EG11221].
UniProt Comments for G6PD
Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.
Product References and Citations for G6PD recombinant protein
Huang Y., et al. (2008) Mol Genet Metab. 93 (1):44-53.; Zimny A., et al. (2003) Pol Arch Med Wewn. 110 (5):1327-33;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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