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GAD1 elisa kit :: Mouse glutamate decarboxylase 1 (brain, 67kDa) ELISA Kit

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Catalog # MBS925594
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

glutamate decarboxylase 1 (brain, 67kDa) (GAD1), ELISA Kit

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Also Known As

Mouse Glutamate decarboxylase 1, GAD1 ELISA Kit

Product Synonym Names
Mouse Glutamate decarboxylase 1 (GAD1) ELISA kit; FLJ45882; GAD; SCP; glutamate decarboxylase 1; glutamate decarboxylase 1 (brain; 67kDa)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS925594 Testing Data
Sequence Length
593
3D Structure
ModBase 3D Structure for P48318
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse GAD1. No significant cross-reactivity or interference between mouse GAD1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
6.25 pg/ml-400 pg/ml
Sensitivity
<1.56 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GAD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGAD1purchase
MBS925594 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutamate decarboxylase 1 (brain, 67kDa) (GAD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GAD1. The ELISA analytical biochemical technique of the MBS925594 kit is based on GAD1 antibody-GAD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GAD1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of GAD1 elisa kit
GAD1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS925594. Click to request current manual
NCBI/Uniprot data below describe general gene information for GAD1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
66,648 Da
NCBI Official Full Name
glutamate decarboxylase 1
NCBI Official Synonym Full Names
glutamate decarboxylase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
EP10; GAD25; GAD44; GAD67; Gad-1
  [Similar Products]
NCBI Protein Information
glutamate decarboxylase 1; GAD-67; glutamic acid decarboxylase 1; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE)
UniProt Protein Name
Glutamate decarboxylase 1
UniProt Synonym Protein Names
67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
Gad67; GAD-67  [Similar Products]
UniProt Entry Name
DCE1_MOUSE
NCBI Summary for GAD1
The protein encoded by this gene is an enzyme that catalyzes the production of gamma-aminobutyric acid from L-glutamic acid. Alternative splicing of this gene results in two products, the predominant 67 kDa isoform and a smaller 25 kDa isoform. The smaller isoform is not thought to retain catalytic activity. [provided by RefSeq, Aug 2015]
UniProt Comments for GAD1
GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Other Amino Acids Metabolism - taurine and hypotaurine; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; EC 4.1.1.15; Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - beta-alanine; Lyase

Cellular Component: mitochondrion; axon; cytoplasm; presynaptic active zone; synapse; cell cortex; intracellular; nerve terminal

Molecular Function: glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; lyase activity; carboxy-lyase activity; protein N-terminus binding; catalytic activity; pyridoxal phosphate binding

Biological Process: gamma-aminobutyric acid biosynthetic process; carboxylic acid metabolic process; social behavior; neurotransmitter biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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