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Gal-7 elisa kit :: Human Galectin 7 (Gal-7) ELISA Kit

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Catalog # MBS701810 (SPECIAL PROMOTIONAL PRICING for a limited time)
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  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Galectin 7 (Gal-7), ELISA Kit

Popular Item
Full Product Name

Human Galectin 7, Gal-7 ELISA Kit

Product Synonym Names
Human Galectin 7 (Gal-7) ELISA Kit; Galectin 7 (Gal-7)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS701810 Testing Data
OMIM
600615
3D Structure
ModBase 3D Structure for P47929
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human Gal-7. No significant cross-reactivity or interference between Human Gal-7 and analogues was observed.
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Gal-7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGal-7purchase
MBS701810 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Galectin 7 (Gal-7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Gal-7. The ELISA analytical biochemical technique of the MBS701810 kit is based on Gal-7 antibody-Gal-7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Gal-7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Gal-7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Gal-7 elisa kit
Introduction: Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Galectin 7 is thought to be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Galectin 7 has been implicated in apoptosis and is believed to upregulate MMP9 and as such be involved in the progression of tumorigenesis. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gal-7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Gal-7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Gal-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Gal-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of Gal-7 elisa kit
Gal-7 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS701810. Click to request current manual
NCBI/Uniprot data below describe general gene information for Gal-7. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15,075 Da
NCBI Official Full Name
galectin-7
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 7B
NCBI Official Symbol
LGALS7B  [Similar Products]
NCBI Official Synonym Symbols
PI7; GAL7; Gal-7; HKL-14; LGALS7
  [Similar Products]
NCBI Protein Information
galectin-7; galectin 7B; galectin-7B; p53-induced protein 1; keratinocyte lectin 14; p53-induced gene 1 protein; lectin galactoside-binding soluble 7
UniProt Protein Name
Galectin-7
UniProt Synonym Protein Names
HKL-14; PI7; p53-induced gene 1 protein
UniProt Gene Name
LGALS7  [Similar Products]
UniProt Synonym Gene Names
PIG1
LGALS7B; Gal-7  [Similar Products]
UniProt Entry Name
LEG7_HUMAN
NCBI Summary for Gal-7
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridization studies indicate that this lectin is specifically expressed in keratinocytes and found mainly in stratified squamous epithelium. A duplicate copy of this gene (GeneID:3963) is found adjacent to, but on the opposite strand on chromosome 19. [provided by RefSeq, Jul 2008]
UniProt Comments for Gal-7
Function: Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.1 PublicationManual assertion based on experiment in:Ref.6
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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