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Gamma secretase elisa kit :: Mouse Gamma secretase ELISA Kit

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Catalog # MBS720137
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Gamma secretase, ELISA Kit

Popular Item
Also Known As

Mouse Gamma secretase ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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OMIM
607839
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of GSEC. No significant cross-reactivity or interference between GSEC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GSEC and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 ng/mL.
Intended Uses
This GSEC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GSEC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Gamma secretase elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGamma secretasepurchase
MBS720137 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gamma secretase, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Gamma secretase. The ELISA analytical biochemical technique of the MBS720137 kit is based on Gamma secretase antibody-Gamma secretase antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Gamma secretase antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Gamma secretase. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Gamma secretase elisa kit
Principle of the assay: GSEC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GSEC antibody and an GSEC-HRP conjugate. The assay sample and buffer are incubated together with GSEC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSEC concentration since GSEC from samples and GSEC-HRP conjugate compete for the anti-GSEC antibody binding site. Since the number of sites is limited, as more sites are occupied by GSEC from the sample, fewer sites are left to bind GSEC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSEC concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of Gamma secretase elisa kit
Gamma secretase elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS720137. Click to request current manual
NCBI/Uniprot data below describe general gene information for Gamma secretase. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
80,474 Da
NCBI Official Full Name
glucan (1,4-alpha-), branching enzyme 1 (glycogen branching enzyme, Andersen disease, glycogen storage disease type IV), isoform CRA_b
NCBI Official Synonym Full Names
glucan (1,4-alpha-), branching enzyme 1
NCBI Official Symbol
NCBI Official Synonym Symbols
GBE; APBD; GSD4
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NCBI Protein Information
1,4-alpha-glucan-branching enzyme; brancher enzyme; glycogen branching enzyme; glycogen-branching enzyme; amylo-(1,4 to 1,6) transglucosidase; amylo-(1,4 to 1,6) transglycosylase
UniProt Protein Name
1,4-alpha-glucan-branching enzyme
UniProt Synonym Protein Names
Brancher enzyme; Glycogen-branching enzyme
UniProt Gene Name
UniProt Entry Name
GLGB_HUMAN
NCBI Summary for Gamma secretase
The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq, Jul 2008]
UniProt Comments for Gamma secretase
GBE1: a monomeric enzyme that functions in glycogen symthesis by catalyzing the formation of alpha 1,6- glucosidic linkages.Required for sufficient glycogen accumulation. The alpha 1-6 branches of glycogen play an important role in increasing the solubility of the molecule and, consequently, in reducing the osmotic pressure within cells. It is most highly expressed in liver and muscle. Deficiency can result in glycogen storage disease IV (Andersen's disease).

Protein type: EC 2.4.1.18; Transferase; Carbohydrate Metabolism - starch and sucrose

Chromosomal Location of Human Ortholog: 3p12.3

Cellular Component: cytosol

Molecular Function: cation binding; 1,4-alpha-glucan branching enzyme activity; hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process: glycogen metabolic process; generation of precursor metabolites and energy; glycogen biosynthetic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis

Disease: Polyglucosan Body Disease, Adult Form; Glycogen Storage Disease Iv
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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