• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-GARS antibody :: Mouse anti-Human Glycyl-TRNA Synthetase Antibody

Scan QR to view Datasheet
Catalog # MBS140491
Unit / Price
  0.005 mg  /  $140 +1 FREE 8GB USB
  0.02 mg  /  $205 +1 FREE 8GB USB
  0.1 mg  /  $505 +1 FREE 8GB USB
anti-GARS antibody
Product Name

Glycyl-TRNA Synthetase (GARS), Antibody

Popular Item
Also Known As

Mouse Anti Human Glycyl-TRNA Synthetase

Product Synonym Names
GARS Antibody; Glycyl-TRNA Synthetase, Mouse Anti Human; Glycine--tRNA ligase; Diadenosine tetraphosphate synthetase; AP-4-A synthetase; Glycyl-tRNA synthetase; GlyRS; GARS; CMT2D; DSMAV; HMN5; SMAD1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
739
OMIM
600287
Isotype
Mouse IgG1 heavy chain and k light chain.
Clone Number
PAT4E10AT
Host
Mouse
Species Reactivity
Human
Purity/Purification
GARS antibody was purified from mouse ascitic fluids by protein -A affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Sterile filtered colorless solution.
Type
Mouse Anti Human Monoclonal
Immunogen
Anti-human GARS mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GARS amino acids 43-289 purified from E Coli.
Preparation and Storage
Storage: For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.

Prevent freeze thaw cycles.

Stability: 12 months at -20°C. 1 month at 4°C.
Other Notes
Small volumes of anti-GARS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GARS antibody
GARS is an (alpha)2 dimer which is a member of the class II family of tRNA synthetases. GARS is a glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases which charge tRNAs with their cognate amino acids. GARS catalyzes the attachment of glycine to tRNA(Gly). In addition, GARS is able to produce diadenosine tetraphosphate (Ap4A), which is a universal pleiotropic signaling molecule required for cell regulation pathways, by direct condensation of two ATPs. GARS has been demonstrated to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.
Applications Tested/Suitable for anti-GARS antibody
GARS antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Application Notes for anti-GARS antibody
Recommended starting dilution is 1:500
NCBI/Uniprot data below describe general gene information for GARS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
glycyl tRNA synthetase
NCBI Official Synonym Full Names
glycyl-tRNA synthetase
NCBI Official Symbol
NCBI Official Synonym Symbols
HMN5; CMT2D; DSMAV; GlyRS; SMAD1
  [Similar Products]
NCBI Protein Information
glycine--tRNA ligase
UniProt Protein Name
Glycine--tRNA ligase
UniProt Synonym Protein Names
Diadenosine tetraphosphate synthetase; AP-4-A synthetase; Glycyl-tRNA synthetase; GlyRS
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
AP-4-A synthetase; GlyRS  [Similar Products]
UniProt Entry Name
SYG_HUMAN
UniProt Comments for GARS
GARS: Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Defects in GARS are the cause of Charcot-Marie-Tooth disease type 2D (CMT2D). CMT2D is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2D is characterized by a more severe phenotype in the upper extremities (severe weakness and atrophy, absence of tendon reflexes) than in the lower limbs. CMT2D inheritance is autosomal dominant. Defects in GARS are a cause of distal hereditary motor neuronopathy type 5A (HMN5A); also known as distal hereditary motor neuropathy type V (DSMAV). A disorder characterized by distal muscular atrophy mainly affecting the upper extremities, in contrast to other distal motor neuronopathies. These constitute a heterogeneous group of neuromuscular diseases caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: EC 6.1.1.14; Ligase; Mitochondrial; Translation

Chromosomal Location of Human Ortholog: 7p15

Cellular Component: axon; cytoplasm; cytosol; mitochondrial matrix; nucleoplasm

Molecular Function: bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity; glycine-tRNA ligase activity; protein dimerization activity

Biological Process: diadenosine tetraphosphate biosynthetic process; glycyl-tRNA aminoacylation; tRNA aminoacylation for protein translation

Disease: Charcot-marie-tooth Disease, Axonal, Type 2d; Neuronopathy, Distal Hereditary Motor, Type Va
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withanti-GARS antibody
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000