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GasR elisa kit :: Human GasR ELISA Kit

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Catalog # MBS2503460
Unit / Price DISCONTINUED
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Typical Testing Data/Standard Curve (for reference only)
Product Name

GasR, ELISA Kit

Popular Item
Full Product Name

Human GasR (Gastrin Receptor) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
118445
3D Structure
ModBase 3D Structure for P32239
Species Reactivity
Specificity
This kit recognizes natural and recombinant Human GasR. No significant cross-reactivity or interference between Human GasR and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GasR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGasRpurchase
MBS2503460 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GasR, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GasR. The ELISA analytical biochemical technique of the MBS2503460 kit is based on GasR antibody-GasR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GasR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GasR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GasR elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GasR concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GasR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GasR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GasR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GasR. You can calculate the concentration of GasR in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of GasR elisa kit
GasR elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2503460. Click to request current manual
NCBI/Uniprot data below describe general gene information for GasR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
41,504 Da
NCBI Official Full Name
gastrin/cholecystokinin type B receptor
NCBI Official Synonym Full Names
cholecystokinin B receptor
NCBI Official Symbol
CCKBR  [Similar Products]
NCBI Official Synonym Symbols
GASR; CCK-B; CCK2R
  [Similar Products]
NCBI Protein Information
gastrin/cholecystokinin type B receptor; CCK-B receptor; CCK-BR; CCK2 receptor; CCK2-R; cholecystokinin-2 receptor; gastrin receptor
UniProt Protein Name
Gastrin/cholecystokinin type B receptor
UniProt Synonym Protein Names
Cholecystokinin-2 receptor; CCK2-R
UniProt Gene Name
CCKBR  [Similar Products]
UniProt Synonym Gene Names
CCKRB; CCK-B receptor; CCK-BR; CCK2-R  [Similar Products]
UniProt Entry Name
GASR_HUMAN
NCBI Summary for GasR
This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq, Jul 2008]
UniProt Comments for GasR
CCKBR: Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: type B gastrin/cholecystokinin receptor binding; protein binding; gastrin receptor activity; 1-phosphatidylinositol-3-kinase regulator activity; cholecystokinin receptor activity; phosphoinositide phospholipase C activity

Biological Process: gland development; gastric acid secretion; cell proliferation; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; gut development; metabolic process; sensory perception; digestion; positive regulation of cell proliferation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); feeding behavior; regulation of phosphoinositide 3-kinase activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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