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GC elisa kit :: Vitamin D Binding Protein ELISA Kit

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Catalog # MBS564062
Unit / Price
  1x96 Wells  /  $500 +1 FREE 8GB USB
GC elisa kit
Product Name

Vitamin D Binding Protein (GC), ELISA Kit

Popular Item
Also Known As

Human Vitamin D Binding Protein (GC Globulin) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 4; NC_000004.11 (72607410..72649888, complement). Location: 4q12-q13
OMIM
139200
3D Structure
ModBase 3D Structure for P02774
Host
Human
Samples
Immunoperoxidase Assay for Determination of Vitamin D Binding Protein in Human Samples
Intended Uses
The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the VDBP present in samples reacts with the anti-VDBP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-VDBP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound VDBP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of VDBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of VDBP in the test sample. The quantity of VDBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Preparation and Storage
Store at 4 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGCpurchase
MBS564062 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Human Vitamin D Binding Protein (GC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GC. The ELISA analytical biochemical technique of the MBS564062 kit is based on GC antibody-GC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for GC elisa kit
Sample Manual Insert of MBS564062. Click to request current manual
NCBI/Uniprot data below describe general gene information for GC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
52,964 Da[Similar Products]
NCBI Official Full Name
vitamin D-binding protein isoform 1
NCBI Official Synonym Full Names
group-specific component (vitamin D binding protein)
NCBI Official Symbol
NCBI Official Synonym Symbols
DBP; GRD3; VDBG; VDBP; DBP/GC
  [Similar Products]
NCBI Protein Information
vitamin D-binding protein; VDB; gc-globulin; OTTHUMP00000160364; OTTHUMP00000218973; OTTHUMP00000218974; OTTHUMP00000218975; vitamin D-binding alpha-globulin
UniProt Protein Name
Vitamin D-binding protein
UniProt Synonym Protein Names
Gc-globulin; Group-specific component
Protein Family
UniProt Gene Name
UniProt Entry Name
VTDB_HUMAN
NCBI Summary for GC
The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq]
UniProt Comments for GC
GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: extracellular space; lysosomal lumen; extracellular region; cytosol

Molecular Function: vitamin D binding; actin binding; vitamin transporter activity

Biological Process: steroid metabolic process; vitamin transport; vitamin D metabolic process

Disease: Graves Disease, Susceptibility To, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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