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Gcg elisa kit :: Mouse Glucagon ELISA Kit

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Catalog # MBS2886862
Unit / Price
  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Glucagon (Gcg), ELISA Kit

Popular Item
Also Known As

Mouse Glucagon ELISA Kit

Product Synonym Names
Glucagon; Gcg
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2886862 Testing Data
Sequence Length
180
3D Structure
ModBase 3D Structure for P55095
Species Reactivity
Assay Type
Sandwich
Detection Range
15.6-1000 pg/mL
Sensitivity
8.04 pg/mL
Intra-Assay CV
<=4.3%
Inter-Assay CV
<=6.4%
Recovery
102%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Gcg elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGcgpurchase
MBS2886862 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucagon (Gcg) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Gcg. The ELISA analytical biochemical technique of the MBS2886862 kit is based on Gcg antibody-Gcg antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Gcg antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Gcg. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Gcg elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Standard Curve/Testing Data of Gcg elisa kit
Gcg elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS2886862. Click to request current manual
NCBI/Uniprot data below describe general gene information for Gcg. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,906 Da
NCBI Official Full Name
glucagon preproprotein
NCBI Official Synonym Full Names
glucagon
NCBI Official Symbol
NCBI Official Synonym Symbols
Glu; PPG; GLP-1
  [Similar Products]
NCBI Protein Information
glucagon
UniProt Protein Name
Glucagon
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2  [Similar Products]
UniProt Entry Name
GLUC_MOUSE
NCBI Summary for Gcg
This gene encodes glucagon, a pancreatic hormone that counteracts the action of insulin in the bloodstream. The encoded protein is processed to generate glucagon and two other glucagon-like peptides, GLP1 and GLP2. Glucagon stimulates gluconeogenesis, glycogenolysis and lipolysis. GLP1 induces secretion of insulin, suppresses glucagon secretion and inhibits feeding. GLP2 induces intestinal absorption of glucose by stimulating the growth of intestinal cells and preventing apoptosis. [provided by RefSeq, Apr 2015]
UniProt Comments for Gcg
GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family.

Protein type: Secreted, signal peptide; Hormone; Secreted

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: glucagon receptor binding; hormone activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of apoptosis; negative regulation of appetite; positive regulation of cAMP biosynthetic process; positive regulation of histone H3-K4 methylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of protein kinase activity; regulation of insulin secretion; response to starvation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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