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GCSFR elisa kit :: Mouse GCSFR ELISA Kit

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Catalog # MBS2509006
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Typical Testing Data/Standard Curve (for reference only)
Product Name

GCSFR, ELISA Kit

Popular Item
Full Product Name

Mouse GCSFR (Colony Stimulating Factor Receptor, Granulocyte) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
138971
3D Structure
ModBase 3D Structure for Q99062
Species Reactivity
Specificity
This kit recognizes natural and recombinant Mouse GCSFR. No significant cross-reactivity or interference between Mouse GCSFR and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GCSFR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GCSFRpurchase
MBS2509006 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GCSFR, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GCSFR. The ELISA analytical biochemical technique of the MBS2509006 kit is based on GCSFR antibody-GCSFR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GCSFR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GCSFR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GCSFR elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse GCSFR concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GCSFR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GCSFR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GCSFR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GCSFR. You can calculate the concentration of GCSFR in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of GCSFR elisa kit
GCSFR elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2509006. Click to request current manual
NCBI/Uniprot data below describe general gene information for GCSFR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
86,609 Da
NCBI Official Full Name
granulocyte colony-stimulating factor receptor isoform d
NCBI Official Synonym Full Names
colony stimulating factor 3 receptor (granulocyte)
NCBI Official Symbol
CSF3R  [Similar Products]
NCBI Official Synonym Symbols
CD114; GCSFR
  [Similar Products]
NCBI Protein Information
granulocyte colony-stimulating factor receptor; CD114 antigen; G-CSF receptor; G-CSF-R
UniProt Protein Name
Granulocyte colony-stimulating factor receptor
UniProt Synonym Protein Names
CD_antigen: CD114
UniProt Gene Name
CSF3R  [Similar Products]
UniProt Synonym Gene Names
GCSFR; G-CSF receptor; G-CSF-R  [Similar Products]
UniProt Entry Name
CSF3R_HUMAN
NCBI Summary for GCSFR
The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq, Aug 2010]
UniProt Comments for GCSFR
GCSFR: the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variant isoforms have been described.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p35-p34.3

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; receptor activity

Biological Process: neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; cell adhesion; signal transduction

Disease: Neutrophilia, Hereditary
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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