• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GDF-5 recombinant protein

Scan QR to view Datasheet
Catalog # MBS203335
Unit / Price
  0.1 mg  /  $310 +1 FREE 8GB USB
  0.5 mg  /  $720 +1 FREE 8GB USB
SDS-Page
Product Name

GDF-5, Recombinant Protein

Also Known As

GDF-5, 382-501aa, Human, His tag, E Coli

Product Synonym Names
Growth differentiation factor 5; Growth differentiation factor 5; BMP14; CDMP1; LAP4; OS5; SYNS2; Growth differentiation factor 5 Cartilage derived morphogenetic protein 1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
501
Sequence
MGSSHHHHHH SSGLVPRGSH MAPLATRQGK RPSKNLKARC SRKALHVNFK DMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC R
OMIM
X80915 Genomic DNA
3D Structure
ModBase 3D Structure for P43026
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 10 mM Sodium citrate pH 3.5, 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of GDF-5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GDF-5 recombinant protein
Growth differentiation factor 5 (GDF-5) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This protein plays a role in chondrogenesis and chondrocyte metabolism, tendon and ligament tissue formation, and bone repair. It also increases the survival of neurones that respond to a neurotransmitter called dopamine, and is a potential therapeutic molecule associated with Parkinson's disease. GDF-5, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E Coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Categories/Family for GDF-5 recombinant protein
Applications Tested/Suitable for GDF-5 recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for GDF-5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
15.8 kDa (141aa), confirmed by MALDI-TOF.
NCBI Official Full Name
growth/differentiation factor 5 preproprotein
NCBI Official Synonym Full Names
growth differentiation factor 5
NCBI Official Symbol
NCBI Official Synonym Symbols
OS5; LAP4; BDA1C; BMP14; CDMP1; LAP-4; SYM1B; SYNS2; BMP-14
  [Similar Products]
NCBI Protein Information
growth/differentiation factor 5
UniProt Protein Name
Growth/differentiation factor 5
UniProt Synonym Protein Names
Bone morphogenetic protein 14; BMP-14; Cartilage-derived morphogenetic protein 1; CDMP-1; Lipopolysaccharide-associated protein 4; LAP-4; LPS-associated protein 4; Radotermin
UniProt Gene Name
UniProt Synonym Gene Names
BMP14; CDMP1; GDF-5; BMP-14; CDMP-1; LAP-4; LPS-associated protein 4  [Similar Products]
NCBI Summary for GDF-5
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates the development of numerous tissue and cell types, including cartilage, joints, brown fat, teeth, and the growth of neuronal axons and dendrites. Mutations in this gene are associated with acromesomelic dysplasia, brachydactyly, chondrodysplasia, multiple synostoses syndrome, proximal symphalangism, and susceptibility to osteoarthritis. [provided by RefSeq, Aug 2016]
UniProt Comments for GDF-5
Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed:24098149, PubMed:21976273, PubMed:15530414, PubMed:25092592). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed:21976273). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205).
Product References and Citations for GDF-5 recombinant protein
Feng G., et al. (2008). Growth Factor. 26 (3):132-42; Sullivan AM., et al. (2005). J Anat. 207 (3):219-26.;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000