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GDF11 elisa kit :: Human growth differentiation factor 11 ELISA Kit

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Catalog # MBS939778
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

growth differentiation factor 11 (GDF11), ELISA Kit

Popular Item
Also Known As

Human Growth/differentiation factor 11, GDF11 ELISA Kit

Product Synonym Names
Human Growth/differentiation factor 11 (GDF11) ELISA kit; BMP-11; BMP11; bone morphogenetic protein 11; growth differentiation factor 11
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS939778 Testing Data
Sequence Length
407
OMIM
603936
3D Structure
ModBase 3D Structure for O95390
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human GDF11. No significant cross-reactivity or interference between human GDF11 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
28 pg/ml-1800 pg/ml
Sensitivity
7 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GDF11 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGDF11purchase
MBS939778 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the growth differentiation factor 11 (GDF11) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GDF11. The ELISA analytical biochemical technique of the MBS939778 kit is based on GDF11 antibody-GDF11 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GDF11 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GDF11. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Intended use of this ELISA kit is quantitative detection of human growth differentiation factor 11 (GDF11) in serum, plasma, and tissue homogenates.

GDF11’s alternative name in NCBI and UniProt data bases is bone morphogenetic protein 11 (BMP11).

GDF11 belongs to family of bone morphogenetic proteins which regulate cell growth and differentiation in embryonic and adult tissues.

GDF11 test kit is designed as sandwich-type ELISA and concentration of GDF11 is measured using pair of antibodies specific to target protein.

Product contains microwell plate pre-coated with capture antibodies against GDF11, and all reagents required for its quantification, including sample and reagent diluents, and wash solution.

Assay kit provides colorimetric detection of GDF11 using biotinylated detector antibodies against GDF11, avidin conjugated to HRP, and TMB as its substrate solution.

Minimal detectable concentration of ELISA kit is 7.0pg/mL and its reportable range is 28-1800pg/mL; concentrated calibrator’s preparation for standard curve is supplied in lyophilized form and requires dilution for making working levels of calibrators.

Intra- and inter- assay CV values, reported as kit’s precision characteristics, are not greater than 8% and 10%, respectively.

Assay doesn’t show significant cross-reactivity with analogous molecules.

ELISA kit and its reagents and components require refrigeration and must be stored at 2-8¬įC until indicated expiration date.
Related Product Information for
GDF11 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GDF11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GDF11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GDF11 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GDF11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of GDF11 elisa kit
GDF11 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS939778. Click to request current manual
NCBI/Uniprot data below describe general gene information for GDF11. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,091 Da
NCBI Official Full Name
growth/differentiation factor 11
NCBI Official Synonym Full Names
growth differentiation factor 11
NCBI Official Symbol
GDF11  [Similar Products]
NCBI Official Synonym Symbols
BMP11; BMP-11
  [Similar Products]
NCBI Protein Information
growth/differentiation factor 11; GDF-11; bone morphogenetic protein 11
UniProt Protein Name
Growth/differentiation factor 11
UniProt Synonym Protein Names
Bone morphogenetic protein 11; BMP-11
UniProt Gene Name
GDF11  [Similar Products]
UniProt Synonym Gene Names
BMP11; GDF-11; BMP-11  [Similar Products]
UniProt Entry Name
GDF11_HUMAN
NCBI Summary for GDF11
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development. [provided by RefSeq, Jul 2008]
UniProt Comments for GDF11
GDF11: Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding

Biological Process: regulation of apoptosis; negative regulation of cell proliferation; nervous system development; negative regulation of cell differentiation; camera-type eye morphogenesis; spinal cord anterior/posterior patterning; ureteric bud development; regulation of MAPKKK cascade; pancreas development; cell maturation; mesoderm development; palate development; skeletal development; cell development; metanephros development; growth
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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