• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GDNF elisa kit :: Human GDNF ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2512291
Unit / Price
Scan QR to view Datasheet
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

GDNF, ELISA Kit

Popular Item
Full Product Name

Human GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
209880
3D Structure
ModBase 3D Structure for P39905
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GDNF. No significant cross-reactivity or interference between Human GDNF and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GDNF and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GDNF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GDNFpurchase
MBS2512291 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GDNF, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GDNF. The ELISA analytical biochemical technique of the MBS2512291 kit is based on GDNF antibody-GDNF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GDNF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GDNF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GDNF elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GDNF in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDNF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GDNF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GDNF. You can calculate the concentration of Human GDNF in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of GDNF elisa kit
GDNF elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2512291. Click to request current manual
NCBI/Uniprot data below describe general gene information for GDNF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,123 Da
NCBI Official Full Name
glial cell line-derived neurotrophic factor isoform 4 preproprotein
NCBI Official Synonym Full Names
glial cell derived neurotrophic factor
NCBI Official Symbol
NCBI Official Synonym Symbols
ATF1; ATF2; HSCR3; HFB1-GDNF
  [Similar Products]
NCBI Protein Information
glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor
UniProt Protein Name
Glial cell line-derived neurotrophic factor
UniProt Synonym Protein Names
Astrocyte-derived trophic factor; ATF
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
hGDNF; ATF  [Similar Products]
UniProt Entry Name
GDNF_HUMAN
NCBI Summary for GDNF
This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]
UniProt Comments for GDNF
GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5p13.1-p12

Cellular Component: extracellular region

Molecular Function: protein homodimerization activity; growth factor activity; receptor binding

Biological Process: axon guidance; nervous system development; positive regulation of dopamine secretion; peristalsis; adult locomotory behavior; mRNA stabilization; regulation of dopamine uptake; positive regulation of monooxygenase activity; signal transduction; enteric nervous system development; sympathetic nervous system development; ureteric bud branching; regulation of gene expression; induction of an organ; positive regulation of cell proliferation; postganglionic parasympathetic nervous system development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; postsynaptic membrane organization; positive regulation of cell differentiation; metanephros development; neural crest cell migration; neurite development; negative regulation of apoptosis

Disease: Hirschsprung Disease, Susceptibility To, 3; Central Hypoventilation Syndrome, Congenital; Pheochromocytoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000