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GGT1 elisa kit :: Human Gamma-glutamyltranspeptidase 1 ELISA Kit

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Catalog # MBS762013
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Gamma-glutamyltranspeptidase 1 (GGT1), ELISA Kit

Popular Item
Full Product Name

Human Gamma-glutamyltranspeptidase 1 ELISA Kit

Product Synonym Names
gammaGT1/GGT1 ( (Gamma Glutamyltransferase 1))/GGT/Leukotriene-C4 hydrolase/Glutathione hydrolase 1/Gamma-glutamyltranspeptidase 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
231950
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of GGT1. No significant cross-reactivity or interference between GGT1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
1.563-100ng/ml
Sensitivity
<0.938ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GGT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GGT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GGT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GGT1purchase
MBS762013 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gamma-glutamyltranspeptidase 1 (GGT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GGT1. The ELISA analytical biochemical technique of the MBS762013 kit is based on GGT1 antibody-GGT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GGT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GGT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GGT1 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GGT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- GGT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GGT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GGT1 can be calculated.

Typical Testing Data/Standard Curve (for reference only) of GGT1 elisa kit
GGT1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for GGT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,080 Da
NCBI Official Full Name
gamma-glutamyltranspeptidase 1
NCBI Official Synonym Full Names
gamma-glutamyltransferase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
GGT; GTG; CD224; GGT 1; D22S672; D22S732
  [Similar Products]
NCBI Protein Information
gamma-glutamyltranspeptidase 1
UniProt Protein Name
Gamma-glutamyltranspeptidase 1
UniProt Synonym Protein Names
Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13
UniProt Gene Name
UniProt Synonym Gene Names
GGT; GGT 1  [Similar Products]
UniProt Entry Name
GGT1_HUMAN
NCBI Summary for GGT1
The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]
UniProt Comments for GGT1
GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - selenoamino acid; Membrane protein, integral; Other Amino Acids Metabolism - cyanoamino acid; EC 3.4.19.14; Other Amino Acids Metabolism - glutathione; EC 3.4.19.13; EC 2.3.2.2; Transferase

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: anchored to external side of plasma membrane; extracellular space; integral to membrane; plasma membrane

Molecular Function: gamma-glutamyltransferase activity; protein binding

Biological Process: amino acid metabolic process; arachidonic acid metabolic process; cysteine biosynthetic process; glutamate metabolic process; glutathione biosynthetic process; glutathione catabolic process; glutathione metabolic process; leukotriene biosynthetic process; leukotriene metabolic process; regulation of immune system process; regulation of inflammatory response; spermatogenesis; xenobiotic metabolic process; zymogen activation

Disease: Glutathionuria
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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