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GGT1 elisa kit :: Porcine Gamma Glutamyltransferase 1 ELISA Kit

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Catalog # MBS045056
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GGT1 elisa kit
Product Name

Gamma Glutamyltransferase 1 (GGT1), ELISA Kit

Popular Item
Full Product Name

Porcine Gamma Glutamyltransferase 1 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Chromosome Location
Chromosome: 22; NC_000022.10 (24979718..25024972). Location: 22q11.23
OMIM
231950
3D Structure
ModBase 3D Structure for P19440
Species Reactivity
Specificity
No significant cross-reactivity or interference between Porcine GGT1 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type
Sandwich
Detection Range
6.25 U/L - 200 U/L.
Sensitivity
1.0 U/L.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GGT1 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine GGT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GGT1 and GGT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GGT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Preparation and Storage
Store all reagents at 2-8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GGT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GGT1purchase
MBS045056 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gamma Glutamyltransferase 1 (GGT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GGT1. The ELISA analytical biochemical technique of the MBS045056 kit is based on GGT1 antibody-GGT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GGT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GGT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GGT1 elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GGT1 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine GGT1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GGT1 and GGT1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GGT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample Manual Insert of MBS045056. Click to request current manual
NCBI/Uniprot data below describe general gene information for GGT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61,410 Da
NCBI Official Full Name
GGT1
NCBI Official Synonym Full Names
gamma-glutamyltransferase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
GGT; GTG; CD224; GGT 1; D22S672; D22S732
  [Similar Products]
NCBI Protein Information
gamma-glutamyltranspeptidase 1; glutamyl transpeptidase; glutathione hydrolase 1; leukotriene-C4 hydrolase; gamma-glutamyl transpeptidase
UniProt Protein Name
Gamma-glutamyltranspeptidase 1
UniProt Synonym Protein Names
Gamma-glutamyltransferase 1; Glutathione hydrolase 1 (EC:3.4.19.13); Leukotriene-C4 hydrolase (EC:3.4.19.14); CD_antigen: CD224Cleaved into the following 2 chains:Gamma-glutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain
UniProt Gene Name
UniProt Synonym Gene Names
GGT; GGT 1  [Similar Products]
UniProt Entry Name
GGT1_HUMAN
NCBI Summary for GGT1
The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Oct 2008]
UniProt Comments for GGT1
GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 2.3.2.2; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - selenoamino acid; Other Amino Acids Metabolism - glutathione; Other Amino Acids Metabolism - cyanoamino acid; EC 3.4.19.13; Lipid Metabolism - arachidonic acid; Transferase; Membrane protein, integral; EC 3.4.19.14

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: anchored to external side of plasma membrane; extracellular space; plasma membrane; integral to membrane

Molecular Function: protein binding; gamma-glutamyltransferase activity

Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; glutathione catabolic process; regulation of inflammatory response; glutathione biosynthetic process; arachidonic acid metabolic process; zymogen activation; spermatogenesis

Disease: Glutathionuria
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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