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GH elisa kit :: Human Anti Growth Hormone Antibody ELISA Kit

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Catalog # MBS726400
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Anti Growth Hormone Antibody (GH), ELISA Kit

Also Known As

Human Anti Growth Hormone Antibody ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
612781
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000ng/mL
Sensitivity
1.0ng/mL
Intended Uses
This Anti-GH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Anti-GH. This ELISA kit for research use only, not for therapeutic or diagnostic applications
!Intended Uses
This Anti-GH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Anti-GH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGHpurchase
MBS726400 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti Growth Hormone Antibody (GH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GH. The ELISA analytical biochemical technique of the MBS726400 kit is based on GH antibody-GH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GH elisa kit
Principle of the Assay: Anti-GH ELISA kit applies the competitive enzyme immunoassay technique utilizing GH antigen and an Anti-GH-HRP conjugate. The assay sample and buffer are incubated together with Anti-GH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-GH concentration since Anti-GH from samples and Anti-GH-HRP conjugate compete for the GH antigen binding site. Since the number of sites is limited, as more sites are occupied by Anti-GH from the sample, fewer sites are left to bind Anti-GH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-GH concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of GH elisa kit
GH elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS726400. Click to request current manual
NCBI/Uniprot data below describe general gene information for GH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,201 Da
NCBI Official Full Name
growth hormone
NCBI Official Synonym Full Names
growth hormone 1
NCBI Official Symbol
NCBI Official Synonym Symbols
GH; GHN; GH-N; hGH-N; IGHD1B
  [Similar Products]
NCBI Protein Information
somatotropin; pituitary growth hormone
UniProt Protein Name
Somatotropin
UniProt Synonym Protein Names
Growth hormone; GH; GH-N; Growth hormone 1; Pituitary growth hormone
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GH; GH-N  [Similar Products]
UniProt Entry Name
SOMA_HUMAN
NCBI Summary for GH
The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008]
UniProt Comments for GH
GH: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A); also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH. Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH. Defects in GH1 are the cause of Kowarski syndrome (KWKS); also known as pituitary dwarfism VI. Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2). IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Hormone

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; growth hormone receptor binding; growth factor activity; prolactin receptor binding; hormone activity; metal ion binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of receptor internalization; positive regulation of JAK-STAT cascade; glucose transport; positive regulation of multicellular organism growth; JAK-STAT cascade; response to estradiol stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein

Disease: Isolated Growth Hormone Deficiency, Type Ia; Isolated Growth Hormone Deficiency, Type Ib; Isolated Growth Hormone Deficiency, Type Ii; Kowarski Syndrome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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