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GHRL elisa kit :: Human Ghrelin ELISA Kit

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Catalog # MBS283919
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $745 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,405 +4 FREE 8GB USB
  10x96-Strip-Wells  /  $6,425 +8 FREE 8GB USB
GHRL elisa kit
Product Name

Ghrelin (GHRL), ELISA Kit

Popular Item
Full Product Name

Human Ghrelin (GHRL) ELISA Kit

Product Synonym Names
MTLRP; obestatin; OTTHUMP00000207794|OTTHUMP00000207795|OTTHUMP00000207796|OTTHUMP00000207798|ghrelin; growth hormone secretagogue receptor ligand|ghrelin/obestatin|ghrelin/obestatin preprohormone|m
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
AB029434 mRNA
3D Structure
ModBase 3D Structure for Q9UBU3
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GHRL. No significant cross-reactivity or interference between Human GHRL and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GHRL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGHRLpurchase
MBS283919 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ghrelin (GHRL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GHRL. The ELISA analytical biochemical technique of the MBS283919 kit is based on GHRL antibody-GHRL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GHRL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GHRL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GHRL elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GHRL in samples. An antibody specific for GHRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGHRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GHRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GHRL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS283919. Click to request current manual
NCBI/Uniprot data below describe general gene information for GHRL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
9,972 Da
NCBI Official Full Name
appetite-regulating hormone isoform 2 preproprotein
NCBI Official Synonym Full Names
ghrelin and obestatin prepropeptide
NCBI Official Symbol
NCBI Official Synonym Symbols
MTLRP
  [Similar Products]
NCBI Protein Information
appetite-regulating hormone
UniProt Protein Name
Appetite-regulating hormone
UniProt Synonym Protein Names
Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MTLRP; Ghrelin  [Similar Products]
NCBI Summary for GHRL
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]
UniProt Comments for GHRL
ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach

Protein type: Apoptosis; Cell cycle regulation; Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: axon; endoplasmic reticulum lumen; extracellular region; extracellular space

Molecular Function: G-protein coupled receptor binding; ghrelin receptor binding; growth hormone-releasing hormone activity; protein binding; protein tyrosine kinase activator activity

Biological Process: actin polymerization or depolymerization; activation of MAPK activity; adult feeding behavior; cartilage development; cortisol secretion; decidualization; dendrite development; elevation of cytosolic calcium ion concentration; excitatory postsynaptic potential; G-protein coupled receptor signaling pathway; gastric acid secretion; glucose metabolic process; growth hormone secretion; hormone-mediated signaling pathway; negative regulation of angiogenesis; negative regulation of apoptosis; negative regulation of circadian sleep/wake cycle, REM sleep; negative regulation of endothelial cell proliferation; negative regulation of inflammatory response; negative regulation of insulin secretion; negative regulation of interleukin-1 beta production; negative regulation of interleukin-6 biosynthetic process; negative regulation of locomotion; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of appetite; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of corticotropin secretion; positive regulation of cortisol secretion; positive regulation of growth hormone secretion; positive regulation of growth rate; positive regulation of insulin secretion; positive regulation of multicellular organism growth; positive regulation of synaptogenesis; regulation of cell proliferation; regulation of response to food; regulation of transmission of nerve impulse; response to electrical stimulus; response to estrogen; response to hormone

Disease: Obesity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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