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GHRP-Ghrelin elisa kit :: Human growth hormone releasing peptide-Ghrelin ELISA Kit

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Catalog # MBS733049
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin), ELISA Kit

Popular Item
Full Product Name

Human growth hormone releasing peptide-Ghrelin ELISA Kit

Product Synonym Names
Human growth hormone releasing peptide-Ghrelin ELISA Kit; growth hormone releasing peptide-Ghrelin; growth hormone releasing peptide-Ghrelin (Human)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of GHRP-Ghrelin. No significant cross-reactivity or interference between GHRP-Ghrelin and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GHRP-Ghrelin and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This GHRP-Ghrelin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GHRP-Ghrelin. This ELISA kit for research use only, not for therapeutic or diagnostic applications
Preparation and Storage
Store all reagents at 2-8 degree C
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GHRP-Ghrelin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGHRP-Ghrelinpurchase
MBS733049 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the growth hormone releasing peptide-Ghrelin (GHRP-Ghrelin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GHRP-Ghrelin. The ELISA analytical biochemical technique of the MBS733049 kit is based on GHRP-Ghrelin antibody-GHRP-Ghrelin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GHRP-Ghrelin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GHRP-Ghrelin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GHRP-Ghrelin elisa kit
Principle of the assay: GHRP-Ghrelin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GHRP-Ghrelin antibody and an GHRP-Ghrelin-HRP conjugate. The assay sample and buffer are incubated together with GHRP-Ghrelin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GHRP-Ghrelin concentration since GHRP-Ghrelin from samples and GHRP-Ghrelin-HRP conjugate compete for the anti-GHRP-Ghrelin antibody binding site. Since the number of sites is limited, as more sites are occupied by GHRP-Ghrelin from the sample, fewer sites are left to bind GHRP-Ghrelin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GHRP-Ghrelin concentration in each sample is interpolated from this standard curve.
Product Categories/Family for GHRP-Ghrelin elisa kit

Typical Testing Data/Standard Curve (for reference only) of GHRP-Ghrelin elisa kit
GHRP-Ghrelin elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS733049. Click to request current manual
NCBI/Uniprot data below describe general gene information for GHRP-Ghrelin. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
ghrelin
NCBI Summary for GHRP-Ghrelin
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]
UniProt Comments for GHRP-Ghrelin
ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach

Protein type: Secreted; Apoptosis; Cell development/differentiation; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 3p26-p25

Cellular Component: extracellular space; axon; endoplasmic reticulum lumen; extracellular region

Molecular Function: growth hormone-releasing hormone activity; G-protein-coupled receptor binding; ghrelin receptor binding; protein tyrosine kinase activator activity

Biological Process: cortisol secretion; negative regulation of circadian sleep/wake cycle, REM sleep; positive regulation of cortisol secretion; hormone-mediated signaling; activation of MAPK activity; response to hormone stimulus; negative regulation of interleukin-6 biosynthetic process; decidualization; positive regulation of multicellular organism growth; negative regulation of insulin secretion; positive regulation of adrenocorticotropic hormone secretion; growth hormone secretion; elevation of cytosolic calcium ion concentration; negative regulation of locomotion; positive regulation of appetite; dendrite development; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of circadian sleep/wake cycle, non-REM sleep; regulation of response to food; gastric acid secretion; glucose metabolic process; positive regulation of insulin secretion; adult feeding behavior; negative regulation of interleukin-1 beta production; regulation of cell proliferation; positive regulation of growth hormone secretion; positive regulation of synaptogenesis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cellular protein metabolic process; response to estrogen stimulus; negative regulation of inflammatory response; cartilage development; actin polymerization and/or depolymerization; negative regulation of endothelial cell proliferation; regulation of excitatory postsynaptic membrane potential; negative regulation of apoptosis

Disease: Obesity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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