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GLDH enzyme :: Glutamate Dehydrogenase Enzyme

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Catalog # MBS682068
Unit / Price
  600 Units  /  $285 +1 FREE 8GB USB
GLDH enzyme
Product Name

Glutamate Dehydrogenase (GLDH), Enzyme

Popular Item
Full Product Name

Glutamate Dehydrogenase (Yeast) (GLDH)

Product Synonym Names
Glutamate dehydrogenase (Yeast); GLDH (yeast)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 10; NC_000010.10 (88809959..88854776, complement). Location: 10q23.3
OMIM
138130
3D Structure
ModBase 3D Structure for P00367
Form/Format
Lyophilized Powder
Specifications
L-Glutamate: NADP+ oxidoreductase (deaminating) (EC 1.4.1.4) from yeast
Contaminant
(as GlDH activity = 100%)
Glucose-6-phosphate dehydrogenase < 0.1 %
Phosphogluconate dehydrogenase < 0.5 %
Glutamate dehydrogenase (NAD+) < 0.1 %
Glutathione reductase < 0.1 %
NADPH oxidase < 0.01 %
Reaction Equation
L-Glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH
Specific Activity
> 10 U/mg protein
(for reduction of alpha-ketoglutarate to L-glutamate at 25 degree C and pH 7.6)
State
Ammonium sulfate free, lyophilized powder of highly purified enzyme
One mg of powder contains the following
Protein 700 +/- 200 ug
K-PO4 300 +/- 300 ug
Preparation
Lyophilized
Preparation and Storage
Lyophilized at -20 degree C.
Other Notes
Small volumes of GLDH enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for GLDH enzyme
NCBI/Uniprot data below describe general gene information for GLDH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61,398 Da
NCBI Official Full Name
glutamate dehydrogenase
NCBI Official Synonym Full Names
glutamate dehydrogenase 1
NCBI Official Symbol
GLUD1  [Similar Products]
NCBI Official Synonym Symbols
GDH; GDH1; GLUD; MGC132003
  [Similar Products]
NCBI Protein Information
glutamate dehydrogenase 1, mitochondrial; GDH 1; OTTHUMP00000020018; glutamate dehydrogenase (NAD(P)+)
UniProt Protein Name
Glutamate dehydrogenase 1, mitochondrial
Protein Family
UniProt Gene Name
GLUD1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
DHE3_HUMAN
NCBI Summary for GLDH
This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans.
UniProt Comments for GLDH
Function: May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate

Catalytic activity: L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.

Enzyme regulation: Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH binding site and activate the enzyme.

Subunit structure: Homohexamer.

Subcellular location: Mitochondrion matrix.

Involvement in disease: Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [

MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [

MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver. Ref.22 Ref.23 Ref.24 Ref.25

Sequence similarities: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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