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GLP-2 elisa kit :: Canine GLP-2 ELISA Kit

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Catalog # MBS2515619
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GLP-2 elisa kit
Product Name

GLP-2, ELISA Kit

Popular Item
Full Product Name

Canine GLP-2(glucagon-like peptide-2)ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2515619 COA
OMIM
603659
Species Reactivity
Specificity
This kit recognizes natural and recombinant Canine GLP-2. No significant cross-reactivity or interference between Canine GLP-2 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Competitive
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GLP-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGLP-2purchase
MBS2515619 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GLP-2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GLP-2. The ELISA analytical biochemical technique of the MBS2515619 kit is based on GLP-2 antibody-GLP-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLP-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLP-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GLP-2 elisa kit
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with GLP-2. During the reaction, GLP-2 in the sample or standard competes with a fixed amount of GLP-2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GLP-2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GLP-2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Sample Manual Insert of MBS2515619. Click to request current manual
NCBI/Uniprot data below describe general gene information for GLP-2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
63,001 Da
NCBI Official Full Name
glucagon-like peptide 2 receptor
NCBI Official Synonym Full Names
glucagon-like peptide 2 receptor
NCBI Official Symbol
GLP2R  [Similar Products]
NCBI Protein Information
glucagon-like peptide 2 receptor; GLP-2 receptor
UniProt Protein Name
Glucagon-like peptide 2 receptor
UniProt Gene Name
GLP2R  [Similar Products]
UniProt Synonym Gene Names
GLP-2 receptor; GLP-2-R; GLP-2R  [Similar Products]
UniProt Entry Name
GLP2R_HUMAN
NCBI Summary for GLP-2
This gene encodes a G protein-coupled receptor that is closely related to the glucagon receptor and binds to glucagon-like peptide-2 (GLP2). Signalling through GLP2 stimulates intestinal growth and increases villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. [provided by RefSeq, Dec 2014]
UniProt Comments for GLP-2
GLP2R: This is a receptor for glucagon-like peptide 2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family.

Protein type: GPCR, family 2; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; glucagon receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; positive regulation of cell proliferation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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