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GLS elisa kit :: Human glutaminase ELISA Kit

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Catalog # MBS2602981
Unit / Price
  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $405 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
Typical Testing Data/Standard Curve
Product Name

glutaminase (GLS), ELISA Kit

Popular Item
Also Known As

Human Glutaminase kidney isoform, mitochondrial, GLS ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
138280
3D Structure
ModBase 3D Structure for O94925
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant GLS concentration.
Assay Type
Sandwich
Detection Range
10 ng/ml-0.156ng/ml
Sensitivity
0.05ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GLS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGLSpurchase
MBS2602981 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutaminase (GLS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GLS. The ELISA analytical biochemical technique of the MBS2602981 kit is based on GLS antibody-GLS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GLS elisa kit
Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GLS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Testing Data/Standard Curve of GLS elisa kit
GLS elisa kit Typical Testing Data/Standard Curve image
Sample Manual Insert of MBS2602981. Click to request current manual
NCBI/Uniprot data below describe general gene information for GLS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
65,460 Da[Similar Products]
NCBI Official Full Name
glutaminase kidney isoform, mitochondrial isoform 2
NCBI Official Synonym Full Names
glutaminase
NCBI Official Symbol
NCBI Official Synonym Symbols
GAC; GAM; KGA; GLS1; AAD20
  [Similar Products]
NCBI Protein Information
glutaminase kidney isoform, mitochondrial; K-glutaminase; glutaminase C; L-glutamine amidohydrolase; glutaminase, phosphate-activated
UniProt Protein Name
Glutaminase kidney isoform, mitochondrial
UniProt Synonym Protein Names
K-glutaminase; L-glutamine amidohydrolase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GLS1; KIAA0838; GLS  [Similar Products]
UniProt Entry Name
GLSK_HUMAN
NCBI Summary for GLS
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
UniProt Comments for GLS
glutaminase: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Belongs to the glutaminase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 3.5.1.2; Hydrolase; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 2q32-q34

Cellular Component: mitochondrion; mitochondrial matrix; cytosol

Molecular Function: protein binding; glutaminase activity

Biological Process: synaptic transmission; glutamate secretion; suckling behavior; neurotransmitter secretion; glutamine catabolic process; neurological control of breathing; glutamate biosynthetic process; amino acid biosynthetic process; protein homotetramerization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with GLS elisa kit
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