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GM2 Ab IgM elisa kit :: Bovine Anti-GM2 (ganglioside) Antibody IgM ELISA Kit

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Catalog # MBS755965
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Anti-GM2 (ganglioside) Antibody IgM (GM2 Ab IgM), ELISA Kit

Also Known As

Bovine Anti-GM2 (ganglioside) Antibody IgM ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Anti-GM2 (ganglioside) Antibody IgM. No significant cross-reactivity or interference between Anti-GM2 (ganglioside) Antibody IgM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Anti-GM2 (ganglioside) Antibody IgM and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GM2 Ab IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGM2 Ab IgMpurchase
MBS755965 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-GM2 (ganglioside) Antibody IgM (GM2 Ab IgM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GM2 Ab IgM. The ELISA analytical biochemical technique of the MBS755965 kit is based on GM2 Ab IgM antibody-GM2 Ab IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GM2 Ab IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GM2 Ab IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GM2 Ab IgM elisa kit
Principle of the assay: Anti-GM2 (ganglioside) Antibody IgM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Anti-GM2 (ganglioside) Antibody IgM antibody and an Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate. The assay sample and buffer are incubated together with Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Anti-GM2 (ganglioside) Antibody IgM concentration since Anti-GM2 (ganglioside) Antibody IgM from samples and Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate compete for the anti-Anti-GM2 (ganglioside) Antibody IgM antibody binding site. Since the number of sites is limited, as more sites are occupied by Anti-GM2 (ganglioside) Antibody IgM from the sample, fewer sites are left to bind Anti-GM2 (ganglioside) Antibody IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-GM2 (ganglioside) Antibody IgM concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of GM2 Ab IgM elisa kit
GM2 Ab IgM elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS755965. Click to request current manual
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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