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GOT1 elisa kit :: Mouse glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) ELISA Kit

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Catalog # MBS904927
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GOT1 elisa kit
Product Name

glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), ELISA Kit

Full Product Name

Mouse Aspartate aminotransferase, cytoplasmic, GOT1 ELISA Kit

Product Synonym Names
Mouse Aspartate aminotransferase; cytoplasmic (GOT1) ELISA kit; GIG18; aspartate aminotransferase 1; growth-inhibiting protein 18; glutamic-oxaloacetic transaminase 1; soluble (aspartate aminotransferase 1)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for P05201
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse GOT1. No significant cross-reactivity or interference between Mouse GOT1 and analogues was observed.
Assay Type
Sandwich
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GOT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GOT1purchase
MBS904927 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GOT1. The ELISA analytical biochemical technique of the MBS904927 kit is based on GOT1 antibody-GOT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GOT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GOT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GOT1 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GOT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GOT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GOT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GOT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS904927. Click to request current manual
NCBI/Uniprot data below describe general gene information for GOT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,248 Da
NCBI Official Full Name
aspartate aminotransferase, cytoplasmic
NCBI Official Synonym Full Names
glutamate oxaloacetate transaminase 1, soluble
NCBI Official Symbol
NCBI Official Synonym Symbols
cCAT; Got-1; cAspAT; AI789014
  [Similar Products]
NCBI Protein Information
aspartate aminotransferase, cytoplasmic; transaminase A; cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; cysteine aminotransferase, cytoplasmic
UniProt Protein Name
Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names
Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
cAspAT; cCAT  [Similar Products]
UniProt Entry Name
AATC_MOUSE
UniProt Comments for GOT1
GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: EC 2.6.1.3; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; EC 2.6.1.1; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; Amino Acid Metabolism - tyrosine

Cellular Component: lysosome; cytoplasm; nerve terminal; nucleus; cytosol

Molecular Function: transferase activity; cysteine transaminase activity; carboxylic acid binding; phosphatidylserine decarboxylase activity; aspartate transaminase activity; transaminase activity; catalytic activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; dicarboxylic acid metabolic process; oxaloacetate metabolic process; glycerol biosynthetic process; glutamate catabolic process to aspartate; fatty acid homeostasis; aspartate biosynthetic process; aspartate metabolic process; biosynthetic process; aspartate catabolic process; amino acid biosynthetic process; glutamate catabolic process to 2-oxoglutarate
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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