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GOT1 protein :: Aspartate Aminotransferase Protein

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Catalog # MBS173082
Unit / Price
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  25000 Units  /  $285 +1 FREE 8GB USB
  100000 Units  /  $715 +1 FREE 8GB USB
GOT1 protein
Product Name

Aspartate Aminotransferase (GOT1), Protein

Popular Item
Full Product Name

Glutamate Oxaloacetate Transmainase

Product Synonym Names
Aspartate Transaminase; glutamate oxaloacetate transaminase; AST; GOT; sGOT; AspAT; ASAT; AAT; serum glutamic oxaloacetic transaminase; Aspartate Aminotransferase (AST, GOT), Porcine Heart - Lyophilized
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS173082 COA
3D Structure
ModBase 3D Structure for P00503
Host
Porcine heart
Purity/Purification
Purified
Form/Format
Powder, lyophilized from sodium phosphate, pH7.0 with pyridoxal-5-phosphate
Pale yellow powder.
EC Number
2.6.1.1
Protein
0.73 mg protein/mg solid (Coomassie),0.93 mg protein/mg sollid (A280)
Activity
320 U/mg (Dimension Clinical Chemistry System)
Unit definition
One unit will catalyze the transamination of one miromole of L-aspartate to a-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8
Specific activity
437 U/mg protein (Coomasie), 341 U/mg protein A 280
Appearance
Pale Yellow powder
Contaminants
CPK: <=0.1%
GLDH: <=0.1
GPT/ALT: <= 0.1%
LDH: < 0.1%
MDH: < 0.01%
Protease
< 0.01% (Equivalent to <0.001 O.D /8 min)
Ammonia
< 0.005 micromole/mg
Reconstitution
>= 1 mg/ml in tris buffered saline, 1% BSA, pH 8.0
Handling
Handle as a potentially hazardous substance.
Preparation and Storage
Store at -20°C.Aliquot and freeze at -20°C after reconstitution.

Storage Note: Protect from moisture.
Other Notes
Small volumes of GOT1 protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GOT1 protein
MyBioSource produces Porcine Heart Aspartate Transferase (AST), also known as Glutamate Oxaloacetate Transaminase (GOT) for research and diagnostic manufacturing uses.

Custom preparations, technical support, bulk quantities and aliquoting available. Single batch sizes from 100,000 kU.

There are two SGOT/AST isoenzymes are present in humans, they have high similarity. GOT1, the cytosolic isoenzyme derives mainly from red blood cells and heart. GOT2, the mitochondrial isoenzyme is predominantly present in liver.
NCBI/Uniprot data below describe general gene information for GOT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Aspartate aminotransferase, cytoplasmic
NCBI Official Symbol
NCBI Official Synonym Symbols
cCAT; CASPAT
  [Similar Products]
NCBI Protein Information
aspartate aminotransferase, cytoplasmic; transaminase A; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; cysteine aminotransferase, cytoplasmic
UniProt Protein Name
Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names
Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
UniProt Gene Name
UniProt Synonym Gene Names
cAspAT; cCAT  [Similar Products]
UniProt Entry Name
AATC_PIG
UniProt Comments for GOT1
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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