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GPBB elisa kit :: Canine Glycogen Phosphorylase lsoenzyme BB ELISA Kit

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Catalog # MBS735391
Unit / Price
  48-Strip-Wells  /  $440 +1 FREE 8GB USB
  96-Strip-Wells  /  $640 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,895 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,415 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Glycogen Phosphorylase lsoenzyme BB (GPBB), ELISA Kit

Popular Item
Also Known As

Canine Glycogen Phosphorylase lsoenzyme BB ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
138550
Species Reactivity
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This GPBB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GPBB. This ELISA kit for research use only, not for therapeutic or diagnostic applications
!Intra-assay Precision
This assay has high sensitivity and excellent specificity for detection of GPBB. No significant cross-reactivity or interference between GPBB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPBB and all the analogues, therefore, cross reaction may still exist in some cases.
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GPBB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGPBBpurchase
MBS735391 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glycogen Phosphorylase lsoenzyme BB (GPBB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPBB. The ELISA analytical biochemical technique of the MBS735391 kit is based on GPBB antibody-GPBB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPBB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPBB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GPBB elisa kit
Principle of the assay: GPBB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GPBB. Standards or samples are then added to the microtiter plate wells and GPBB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GPBB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GPBB are added to each well to “sandwich” the GPBB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GPBB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPBB concentration in each sample is interpolated from this standard curve.

Typical Testing Data/Standard Curve (for reference only) of GPBB elisa kit
GPBB elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS735391. Click to request current manual
NCBI/Uniprot data below describe general gene information for GPBB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
96,696 Da
NCBI Official Full Name
glycogen phosphorylase, brain form
NCBI Official Synonym Full Names
phosphorylase, glycogen; brain
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
glycogen phosphorylase, brain form; glycogen phosphorylase B
UniProt Protein Name
Glycogen phosphorylase, brain form
UniProt Gene Name
UniProt Entry Name
PYGB_HUMAN
NCBI Summary for GPBB
The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]
UniProt Comments for GPBB
PYGB: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Belongs to the glycogen phosphorylase family.

Protein type: EC 2.4.1.1; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Phosphorylase; Transferase

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: membrane; cytoplasm

Molecular Function: glycogen phosphorylase activity; pyridoxal phosphate binding

Biological Process: glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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