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GPC-3 elisa kit :: Human Glypican-3, GPC-3 ELISA Kit

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Catalog # MBS161761
Unit / Price
  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $395 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,610 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,135 +3 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Glypican-3, GPC-3, ELISA Kit

Popular Item
Also Known As

Human Glypican-3, GPC-3 ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
597
3D Structure
ModBase 3D Structure for P13265
Species Reactivity
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 10ng/ml
Sensitivity
0.01ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GPC-3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGPC-3purchase
MBS161761 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glypican-3, GPC-3, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPC-3. The ELISA analytical biochemical technique of the MBS161761 kit is based on GPC-3 antibody-GPC-3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPC-3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPC-3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GPC-3 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Glypican-3 (also known as GPC3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human GPC3 antibody. GPC3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human GPC3 Antibody is added and binds to GPC3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GPC3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human GPC3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Typical Testing Data/Standard Curve (for reference only) of GPC-3 elisa kit
GPC-3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS161761. Click to request current manual
NCBI/Uniprot data below describe general gene information for GPC-3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67,049 Da
NCBI Official Full Name
glypican-3
NCBI Official Synonym Full Names
glypican 3
NCBI Official Symbol
NCBI Official Synonym Symbols
OCI-5
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NCBI Protein Information
glypican-3
UniProt Protein Name
Glypican-3
UniProt Synonym Protein Names
Intestinal protein OCI-5
UniProt Gene Name
NCBI Summary for GPC-3
heparan sulfate cell surface proteogycan, may be involved in development of mesodermal tissues, may modulate Igf2 function [RGD, Feb 2006]
UniProt Comments for GPC-3
Inhibits the dipeptidyl peptidase activity of DPP4 (). Cell surface proteoglycan that bears heparan sulfate. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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