• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GPR172A elisa kit :: Human G protein-coupled receptor 172A ELISA Kit

Scan QR to view Datasheet
Catalog # MBS702260
Unit / Price DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Typical Testing Data/Standard Curve (for reference only)
Product Name

G protein-coupled receptor 172A (GPR172A), ELISA Kit

Popular Item
Also Known As

Human riboflavin transporter 3, RFT3 ELISA Kit

Product Synonym Names
Human riboflavin transporter 3 (RFT3) ELISA Kit; D15Ertd747e; GPCR; GPCR41; PAR1; putative G-protein coupled receptor GPCR41; G protein-coupled receptor 172A
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS702260 Testing Data
Sequence Length
445
OMIM
gene 614707
3D Structure
ModBase 3D Structure for Q9HAB3
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human GPR172A. No significant cross-reactivity or interference between Human GPR172A and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Transport
Protein Biological Process 3
Transport
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GPR172A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGPR172Apurchase
MBS702260 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the G protein-coupled receptor 172A (GPR172A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPR172A. The ELISA analytical biochemical technique of the MBS702260 kit is based on GPR172A antibody-GPR172A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPR172A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPR172A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GPR172A elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RFT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RFT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RFT3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RFT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of GPR172A elisa kit
GPR172A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS702260. Click to request current manual
NCBI/Uniprot data below describe general gene information for GPR172A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,777 Da
NCBI Official Full Name
solute carrier family 52, riboflavin transporter, member 2
NCBI Official Synonym Full Names
solute carrier family 52 (riboflavin transporter), member 2
NCBI Official Symbol
SLC52A2  [Similar Products]
NCBI Official Synonym Symbols
PAR1; RFT3; RFVT2; hRFT3; BVVLS2; GPCR41; GPR172A; D15Ertd747e
  [Similar Products]
NCBI Protein Information
solute carrier family 52, riboflavin transporter, member 2; PERV-A receptor 1; riboflavin transporter 3; G protein-coupled receptor 172A; porcine endogenous retrovirus A receptor 1; putative G-protein coupled receptor GPCR41
UniProt Protein Name
Solute carrier family 52, riboflavin transporter, member 2
UniProt Synonym Protein Names
Porcine endogenous retrovirus A receptor 1; PERV-A receptor 1; Protein GPR172A; Riboflavin transporter 3; hRFT3
UniProt Gene Name
SLC52A2  [Similar Products]
UniProt Synonym Gene Names
GPR172A; PAR1; RFT3; PERV-A receptor 1; hRFT3  [Similar Products]
UniProt Entry Name
S52A2_HUMAN
NCBI Summary for GPR172A
This gene encodes a membrane protein which belongs to the riboflavin transporter family. In humans, riboflavin must be obtained by intestinal absorption because it cannot be synthesized by the body. The water-soluble vitamin riboflavin is processed to the coenzymes flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) which then act as intermediaries in many cellular metabolic reactions. Paralogous members of the riboflavin transporter gene family are located on chromosomes 17 and 20. Unlike other members of this family, this gene has higher expression in brain tissue than small intestine. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. Mutations in this gene have been associated with Brown-Vialetto-Van Laere syndrome 2 - an autosomal recessive progressive neurologic disorder characterized by deafness, bulbar dysfunction, and axial and limb hypotonia. [provided by RefSeq, Jul 2012]
UniProt Comments for GPR172A
Function: Riboflavin transporter. Riboflavin transport is Na+-independent but moderately pH-sensitive. Activity is strongly inhibited by riboflavin analogs, such as lumiflavin. Weakly inhibited by flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN). In case of infection by retroviruses, acts as a cell receptor to retroviral envelopes similar to the porcine endogenous retrovirus (PERV-A).3 PublicationsManual assertion based on experiment in:Ref.1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000