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GPX1 elisa kit :: Chicken GPX1 ELISA Kit

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Catalog # MBS2502532
Unit / Price
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  48-Strip-Wells  /  $450 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name


Popular Item
Full Product Name

Chicken GPX1 (Glutathione Peroxidase 1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2502532 COA
3D Structure
ModBase 3D Structure for P07203
Species Reactivity
This kit recognizes natural and recombinantChickenGPX1. No significant cross-reactivity or interference between ChickenGPX1 and analogues was observed.
Serum, Plasma, Biological Fluids
Assay Type
Detection Range
Min: 37.5pg/mL; Max: 4000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GPX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for GPX1purchase
MBS2502532 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GPX1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPX1. The ELISA analytical biochemical technique of the MBS2502532 kit is based on GPX1 antibody-GPX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GPX1 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of ChickenGPX1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GPX1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GPX1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GPX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GPX1.You can calculate the concentration of GPX1in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of GPX1 elisa kit
GPX1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2502532. Click to request current manual
NCBI/Uniprot data below describe general gene information for GPX1. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,289 Da
NCBI Official Full Name
glutathione peroxidase 1 isoform 1
NCBI Official Synonym Full Names
glutathione peroxidase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
glutathione peroxidase 1; GPx-1; GSHPx-1; cellular glutathione peroxidase
UniProt Protein Name
Glutathione peroxidase 1
UniProt Synonym Protein Names
Cellular glutathione peroxidase
UniProt Gene Name
UniProt Synonym Gene Names
GPx-1; GSHPx-1  [Similar Products]
UniProt Entry Name
NCBI Summary for GPX1
This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for GPX1
GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family.

Protein type: EC; Lipid Metabolism - arachidonic acid; Oxidoreductase; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; cytosol; nucleus

Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity; SH3 domain binding

Biological Process: response to nicotine; fat cell differentiation; blood vessel endothelial cell migration; myoblast proliferation; response to toxin; skeletal muscle fiber development; negative regulation of caspase activity; thermoregulation; regulation of gene expression, epigenetic; vasodilation; response to estradiol stimulus; endothelial cell development; regulation of mammary gland epithelial cell proliferation; response to selenium ion; glutathione metabolic process; sensory perception of sound; UV protection; response to symbiotic bacterium; lipoxygenase pathway; hydrogen peroxide catabolic process; response to folic acid; regulation of neuron apoptosis; response to glucose stimulus; arachidonic acid metabolic process; response to gamma radiation; negative regulation of inflammatory response to antigenic stimulus; aging; protein amino acid oxidation; nucleobase, nucleoside and nucleotide metabolic process; skeletal muscle regeneration; angiogenesis involved in wound healing; purine base metabolic process; response to xenobiotic stimulus; positive regulation of protein kinase B signaling cascade; response to reactive oxygen species; triacylglycerol metabolic process; response to hydrogen peroxide; cell redox homeostasis; heart contraction; response to lipid hydroperoxide; purine nucleotide catabolic process; interaction with symbiont; induction of apoptosis by oxidative stress

Disease: Glutathione Peroxidase Deficiency
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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