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GPX1 elisa kit :: Human Glutathione peroxidase 1 ELISA Kit

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Catalog # MBS289983
Unit / Price
  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Glutathione peroxidase 1 (GPX1), ELISA Kit

Popular Item
Also Known As

Human Glutathione peroxidase 1 ELISA Kit

Product Synonym Names
Glutathione peroxidase 1; GPx-1; GSHPx-1; Cellular glutathione peroxidase; GPX1; 1.11.1.9
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS289983 Testing Data
Sequence Length
203
OMIM
138320
3D Structure
ModBase 3D Structure for P07203
Species Reactivity
Specificity
Recombinant and natural Human GPx-1
Assay Type
Sandwich
Detection Range
12.5-800 U/mL
Sensitivity
3.12 U/mL
Intra-Assay CV
<=4.3%
Inter-Assay CV
<=6.7%
Recovery
102%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GPX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGPX1purchase
MBS289983 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glutathione peroxidase 1 (GPX1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPX1. The ELISA analytical biochemical technique of the MBS289983 kit is based on GPX1 antibody-GPX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GPX1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of GPX1 elisa kit
GPX1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS289983. Click to request current manual
NCBI/Uniprot data below describe general gene information for GPX1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,289 Da
NCBI Official Full Name
glutathione peroxidase 1 isoform 1
NCBI Official Synonym Full Names
glutathione peroxidase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
GPXD; GSHPX1
  [Similar Products]
NCBI Protein Information
glutathione peroxidase 1
UniProt Protein Name
Glutathione peroxidase 1
UniProt Synonym Protein Names
Cellular glutathione peroxidase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GPx-1; GSHPx-1  [Similar Products]
UniProt Entry Name
GPX1_HUMAN
NCBI Summary for GPX1
This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for GPX1
GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family.

Protein type: EC 1.11.1.9; Other Amino Acids Metabolism - glutathione; Lipid Metabolism - arachidonic acid; Oxidoreductase

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cytoplasm; cytosol; mitochondrial matrix; mitochondrion

Molecular Function: glutathione peroxidase activity; SH3 domain binding

Biological Process: angiogenesis involved in wound healing; arachidonic acid metabolic process; blood vessel endothelial cell migration; cell redox homeostasis; endothelial cell development; fat cell differentiation; glutathione metabolic process; heart contraction; hydrogen peroxide catabolic process; induction of apoptosis by oxidative stress; interaction with symbiont; lipoxygenase pathway; myoblast proliferation; negative regulation of caspase activity; negative regulation of inflammatory response to antigenic stimulus; nucleobase, nucleoside and nucleotide metabolic process; positive regulation of protein kinase B signaling cascade; protein amino acid oxidation; purine base metabolic process; purine nucleotide catabolic process; regulation of gene expression, epigenetic; regulation of mammary gland epithelial cell proliferation; regulation of neuron apoptosis; response to gamma radiation; response to hydrogen peroxide; response to hydroperoxide; response to reactive oxygen species; response to selenium ion; response to symbiotic bacterium; response to xenobiotic stimulus; sensory perception of sound; skeletal muscle fiber development; skeletal muscle regeneration; thermoregulation; triacylglycerol metabolic process; UV protection; vasodilation

Disease: Glutathione Peroxidase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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