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GPX3 elisa kit :: Human glutathione peroxidase 3 ELISA Kit

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Catalog # MBS704704
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $550 +1 FREE 8GB USB
  96-Strip-Wells  /  $795 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,890 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,465 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

glutathione peroxidase 3 (GPX3), ELISA Kit

Popular Item
Also Known As

Human Glutathione peroxidase 3, GPX3 ELISA Kit

Product Synonym Names
Human Glutathione peroxidase 3 (GPX3) ELISA kit; GPx-P; GSHPx-3; GSHPx-P; extracellular glutathione peroxidase; glutathione peroxidase 3; glutathione peroxidase 3
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS704704 Testing Data
Sequence Length
226
OMIM
138321
3D Structure
ModBase 3D Structure for P22352
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human GPX3. No significant cross-reactivity or interference between human GPX3 and analogues was observed.
Samples
Serum, plasma and tissue homogenates
Assay Type
Sandwich
Detection Range
15.6 uIU/ml -1000 uIU/ml.
Sensitivity
The minimum detectable dose of human GPX3 is typically less than 3.9uIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GPX3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGPX3purchase
MBS704704 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutathione peroxidase 3 (GPX3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPX3. The ELISA analytical biochemical technique of the MBS704704 kit is based on GPX3 antibody-GPX3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPX3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPX3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GPX3 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPX3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of GPX3 elisa kit
GPX3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS704704. Click to request current manual
NCBI/Uniprot data below describe general gene information for GPX3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,552 Da
NCBI Official Full Name
glutathione peroxidase 3
NCBI Official Synonym Full Names
glutathione peroxidase 3 (plasma)
NCBI Official Symbol
NCBI Official Synonym Symbols
GPx-P; GSHPx-3; GSHPx-P
  [Similar Products]
NCBI Protein Information
glutathione peroxidase 3; GPx-3; plasma glutathione peroxidase; extracellular glutathione peroxidase
UniProt Protein Name
Glutathione peroxidase 3
UniProt Synonym Protein Names
Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P  [Similar Products]
UniProt Entry Name
GPX3_HUMAN
NCBI Summary for GPX3
This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]
UniProt Comments for GPX3
Function: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.

Catalytic activity: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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