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granulin elisa kit :: Human Progranulin ELISA Kit

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Catalog # MBS494660-E103
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granulin elisa kit
Product Name

Progranulin, ELISA Kit

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Full Product Name

Progranulin ELISA

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Species Reactivity
Specificity
Commercially available sera from bovine, cat, chicken, dog, donkey, goat, guinea pig, horse, mouse, pig, rabbit, rat and sheep were diluted 1:5 und 1:41 and used as samples in this assay system and the signal intensity was measured. No cross reactivity was detected.
Samples
Human serum and plasma sample
Sensitivity
The analytical sensitivity of the assay yields 0.018 ng/ml (pg/ml; as 2x SD of zero standard in 19fold determination).
Intended Uses
Measurement of human Progranulin in human serum and plasma sample for research use
Preparation and Storage
2 - 8 degree C
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of granulin elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forgranulinpurchase
MBS494660-E103 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Progranulin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing granulin. The ELISA analytical biochemical technique of the MBS494660-E103 kit is based on granulin antibody-granulin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect granulin antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, granulin. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
granulin elisa kit
Background: Progranulin is also known as Granulin Epithelin Precursor, Proepithelin or Acrogranin. It is a 68.5 kDa protein, consisting of 593 amino acids (inclusive Signalpeptid), which appears in vivo in strongly glycosylated form and therefore has a size of approximately 90 kDa (1). Progranulin has seven conserved domains, which are separated by linker sequences. By means of proteolytic cleavage, catalyzed by serine proteases like e.g. elastase, 6-25 kDa large fragments result, that are called Granulines or Epithelines. Progranulin is expressed and secreted in particular in strongly proliferating tissues such as adenoid tissue, spleen, skin epithelium, gastrointestinal mucous membranes, haematopoietic cells and in tumor cells. Until now no specific receptors, which would obtain the effect of Progranulin or the Granulines are known (2, 3). Progranulin seems to be a factor, which affects the wound healing positively. In case of skin lesions the expression is increased in ceratinocytes, in macrophages and in neutrophile cells. Progranulin affects the wound healing indirectly by activation of macrophages and stimulation of angiogenesis in the damaged tissue (4). The physiological effects of Progranulin and Granulines are oppositional. Progranulin can restrain TNF? mediated proinflammatory processes. On the other hand the Granulines seem to stimulate the secretion of pro-inflammatory cytokines. The influence of Progranulin on inflammatory processes could be shown also in arteriosclerotic plaques. Here Progranulin is expressed by smooth muscle cells and affects the migration of monocytes and smooth muscle cells (5). In the central nervous system Progranulin is expressed in microglia and neurons (in neocortical and hippocampal pyramid cells as well as in purkinje cells in the cerebellum). On mRNA level a clear increase of Progranulin expression could be shown during infections or injuries of the CNS, for example in mucopolysaccharidosis type I and IIIB, in viral inflammations of CNS, in amyotrophic lateral sclerosis and in Alzheimer's disease. Beyond that Progranulin seems to be of relevance in the development of sex specific differences during pre- and postnatal development and also for the neural plasticity in adults (6).
Sample Manual Insert of MBS494660-E103. Click to request current manual
NCBI/Uniprot data below describe general gene information for granulin. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
progranulin
NCBI Official Symbol
granulin  [Similar Products]
NCBI Protein Information
progranulin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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