• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GRIN2A elisa kit :: Human glutamate receptor, ionotropic, N-methyl D-aspartate 2A ELISA Kit

Scan QR to view Datasheet
Catalog # MBS906746
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

glutamate receptor, ionotropic, N-methyl D-aspartate 2A (GRIN2A), ELISA Kit

Popular Item
Also Known As

Human Glutamate [NMDA] receptor subunit epsilon-1, GRIN2A ELISA Kit

Product Synonym Names
Human Glutamate [NMDA] receptor subunit epsilon-1 (GRIN2A) ELISA kit; NMDAR2A; NR2A; N-methyl-D-aspartate receptor channel; subunit epsilon-1; N-methyl-D-aspartate receptor subunit 2A; NMDA receptor subtype 2A; OTTHUMP00000160135; OTTHUMP00000174531; glutamate receptor; ionotropic; N-methyl D-aspartate 2A
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS906746 Testing Data
Sequence Length
1464
OMIM
gene 245570
3D Structure
ModBase 3D Structure for Q12879
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of human GRIN2A. No significant cross-reactivity or interference between human GRIN2A and analogues was observed.
Samples
Serum, plasma, tissue homogenates, Cell lysates
Assay Type
Sandwich
Detection Range
15.6 pg/ml -1000 pg/ml.
Sensitivity
The minimum detectable dose of human GRIN2A is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GRIN2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGRIN2Apurchase
MBS906746 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the glutamate receptor, ionotropic, N-methyl D-aspartate 2A (GRIN2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GRIN2A. The ELISA analytical biochemical technique of the MBS906746 kit is based on GRIN2A antibody-GRIN2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GRIN2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GRIN2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GRIN2A elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GRIN2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRIN2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRIN2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRIN2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of GRIN2A elisa kit
GRIN2A elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS906746. Click to request current manual
NCBI/Uniprot data below describe general gene information for GRIN2A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
165,283 Da
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2A isoform 1
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 2A
NCBI Official Symbol
GRIN2A  [Similar Products]
NCBI Official Synonym Symbols
LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A
  [Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; N-methyl-D-aspartate receptor channel, subunit epsilon-1
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2A
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A; NMDAR2A; NR2A; hNR2A
UniProt Gene Name
GRIN2A  [Similar Products]
UniProt Synonym Gene Names
NMDAR2A; GluN2A; NMDAR2A; NR2A; hNR2A  [Similar Products]
UniProt Entry Name
NMDE1_HUMAN
NCBI Summary for GRIN2A
This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Comments for GRIN2A
NMDAR2A: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission.

Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16p13.2

Cellular Component: presynaptic membrane; postsynaptic membrane; synaptic vesicle; cell surface; endoplasmic reticulum; integral to plasma membrane; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; calcium channel activity; N-methyl-D-aspartate selective glutamate receptor activity

Biological Process: startle response; positive regulation of apoptosis; regulation of synaptic plasticity; sensory perception of pain; dopamine metabolic process; synaptic transmission; protein localization; learning and/or memory; transport; response to wounding; visual learning; serotonin metabolic process; response to drug; synaptic transmission, glutamatergic; glutamate signaling pathway; response to amphetamine; sleep; memory; response to ethanol; neurogenesis; ionotropic glutamate receptor signaling pathway; directional locomotion; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; negative regulation of protein catabolic process

Disease: Epilepsy, Focal, With Speech Disorder And With Or Without Mental Retardation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000