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GroEL recombinant protein

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Catalog # MBS203023
Unit / Price
  0.1 mg  /  $255 +1 FREE 8GB USB
  0.5 mg  /  $535 +1 FREE 8GB USB
SDS-Page
Product Name

GroEL, Recombinant Protein

Popular Item
Also Known As

GroEL, 1-548aa, E Coli, E Coli

Product Synonym Names
groL; groEL; mopA; Protein Cpn60; 60 kDa chaperonin; groEL protein; chaperonin groel
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
548
Sequence
MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGI DKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDG TGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAV AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTV ISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDY DREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI RVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNA ATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM
3D Structure
ModBase 3D Structure for P0A6F5
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 25 mM Tris-HCl (pH 7.5) 100mM NaCl, 5mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
E Coli
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of GroEL recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
GroEL recombinant protein
GroEL protein is the major heat shock protein of E Coli and belongs to the chaperonin (HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition. GroEL gene was amplified by PCR from E Coli and cloned into an expression vector. This protein was overexpressed in E Coli and was purified by using conventional chromatography techniques.
Product Categories/Family for GroEL recombinant protein
Applications Tested/Suitable for GroEL recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for GroEL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
57.3kDa (548aa), confirmed by MALDI-TOF.
NCBI Official Full Name
Cpn60 chaperonin GroEL, large subunit of GroESL
NCBI Official Symbol
NCBI Official Synonym Symbols
ECK4137; groEL; JW4103; mopA
  [Similar Products]
NCBI Protein Information
Cpn60 chaperonin GroEL, large subunit of GroESL
UniProt Protein Name
60 kDa chaperonin
UniProt Synonym Protein Names
GroEL protein
UniProt Gene Name
NCBI Summary for GroEL
groL is an essential gene. [More information is available at EcoGene: EG10599]. The Escherichia coli chaperone protein GroEL (Hsp60) and its regulator GroES are necessary for the proper folding of certain proteins , . [More information is available at EcoCyc: EG10599].
UniProt Comments for GroEL
Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.
Product References and Citations for GroEL recombinant protein
Hemmingsen,S.M., et al (1988) Nature. 333 (6171) 330-334; Braig,K., et al (1994) Nature 371 (6498) 578-586; Chen,L and Singler,P.B. (1999) Cell 99 (7) 757-768;

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with GroEL recombinant protein
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