• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GSDMD elisa kit :: Mouse Gasdermin D ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2031861 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $270 +1 FREE 8GB USB
  48-Strip-Wells  /  $505 +1 FREE 8GB USB
  96-Strip-Wells  /  $685 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,770 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,025 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Gasdermin D (GSDMD), ELISA Kit

Popular Item
Also Known As

Gasdermin D (GSDMD) ELISA Kit

Product Synonym Names
GSDMDC1; DF5L; Gasdermin Domain Containing 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
484
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Gasdermin D (GSDMD).
No significant cross-reactivity or interference between Gasdermin D (GSDMD) and analogues was observed.
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.063ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Gasdermin D (GSDMD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Gasdermin D (GSDMD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of GSDMD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forGSDMDpurchase
MBS2031861 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gasdermin D (GSDMD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GSDMD. The ELISA analytical biochemical technique of the MBS2031861 kit is based on GSDMD antibody-GSDMD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GSDMD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GSDMD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
GSDMD elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gasdermin D (GSDMD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gasdermin D (GSDMD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gasdermin D (GSDMD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gasdermin D (GSDMD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for GSDMD elisa kit

Typical Testing Data/Standard Curve (for reference only) of GSDMD elisa kit
GSDMD elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2031861. Click to request current manual
NCBI/Uniprot data below describe general gene information for GSDMD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
52,801 Da
NCBI Official Full Name
gasdermin-D
NCBI Official Synonym Full Names
gasdermin D
NCBI Official Symbol
GSDMD  [Similar Products]
NCBI Official Synonym Symbols
DF5L; DFNA5L; FKSG10; GSDMDC1
  [Similar Products]
NCBI Protein Information
gasdermin-D
UniProt Protein Name
Gasdermin-D
UniProt Synonym Protein Names
Gasdermin domain-containing protein 1
Protein Family
UniProt Gene Name
GSDMD  [Similar Products]
UniProt Synonym Gene Names
DFNA5L; GSDMDC1  [Similar Products]
UniProt Entry Name
GSDMD_HUMAN
NCBI Summary for GSDMD
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]
UniProt Comments for GSDMD
FKSG10: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm

Molecular Function: protein binding

Biological Process: cellular response to extracellular stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000